Polyclonal Antibody Service


Unlike monoclonal antibodies, polyclonal antibodies correspond to multiple epitopes and tend to contain several immunoglobulin subtypes. They can be used as both primary and secondary antibodies in Westerns, ELISAs, and IHCs. 


Elabscience® provides polyclonal antibody services in mouse, rat, rabbit, and other species. Our polyclonal antibody packages include antigen design, peptide synthesis, immunization, purification, ELISA test, and lyophilization. You can simply select your desired packages and send us the specific requirements, and then Elabscience® will offer antibodies with guaranteed quality.


Advantages

Multiple antibody labeling for alternatives: Elabscience® offers multiple antibody labeling for you to choose, including Biotin, HRP, FITC, Cy3, Cy5, APC, etc.

Flexible service portfolio: Different host species (mouse, rat, and rabbit) and purification method (Protein A/G purification/ Antigen Affinity purification) are available for your research demands.

Personalized experiment scheme: Elabscience’s experienced antibody experts will design the personalized experiment scheme according to your specific requirements and applications.


Polyclonal Antibody Services Details

Cat.No.

Service Name

Timeline

Quoted price

Note

S-CA-P001

Peptide synthesis and conjugation

3 weeks

$16*(peptide length)*number+150$

<15AA, purity >85%, 10 mg

S-CA-P002

Protein expression

         6-7weeks

Depend on the protein

 

S-CA-P003

Polyclonal antisera from rabbit

12 weeks

385$

 

S-CA-P004

Polyclonal antisera from mouse

12 weeks

308$

 

S-CA-P005

Polyclonal antisera from rat

12 weeks

385$

 

S-CA-P010

Protein A/G purification

1 week

230$

 

S-CA-P011

Antigen Affinity purification

1 week

385$

 

S-CA-P012

Antibody labeling (Biotin)

1 day

185$

This price is for 1mg marked antibody.
The total price will increase by $120 when the quantity of marked antibody increase by 1mg.

S-CA-P013

Antibody labeling (HRP)

2 days

308$

S-CA-P014

Antibody labeling (FITC)

1 day

185$

S-CA-P015

Antibody labeling (Cy3)

1 day

230$

S-CA-P016

Antibody labeling (Cy5)

1 day

230$

S-CA-P017

Antibody labeling (APC)

2 days

230$

Successful Cases

In this case, customer provides purified antigen, we prepare antibody and identified it by western blot, then determine titer by ELISA and test concentration by BCA.

 

Result

1. Result of Western blot

As the expression information of target protein was not told, we identified the antibody with immunogen. The sample amount is 1.5 μg.


The main band was at the position of 55~72 kDa and a few unpurposed bands showed up. That may due to some unpurposed protein exist among the antigenic protein.


 

2. Result of ELISA

The coated antigen was 0.2 μg. We detected antisera and purified antibody respectively with dilution factor 8×104, 16×104, 32×104, 64×104, 128×104, 256×104, 512×104, 512×104.The 3 times of the blank OD value was regarded as the titer of the antibody.


2.1 Result of antisera

 

Rabbit A

Rabbit B

Dilution factor

 

A

0.050

0.050

0.052

0.049

Blank

 

B

0.057

0.053

0.053

0.055

Negative control 1/104

 

C

0.098

0.107

0.138

0.125

1/256×104

 

D

0.165

0.163

0.215

0.188

1/128×104

 

E

0.293

0.297

0.349

0.356

1/ 64×104

 

F

0.489

0.466

0.616

0.602

1/32×104

 

G

0.894

0.916

1.065

1.088

1/16×104

 

H

1.207

1.262

1.599

1.625

1/8×104

 

 

2.2 Result of purified antibody

 

Rabbit A

Rabbit B

Dilution factor

A

0.06

0.066

0.061

0.062

Blank

B

0.08

0.069

0.072

0.082

Negative control 1/104

C

0.093

0.096

0.125

0.121

1/1024×104

D

0.134

0.127

0.15

0.152

1/512×104

E

0.158

0.163

0.263

0.26

1/256×104

F

0.274

0.275

0.479

0.481

1/128×104

G

0.42

0.422

0.82

0.816

1/ 64×104

H

0.695

0.691

1.356

1.355

1/32×104

The titer of rabbit A is 128×104. The titer of rabbit B is 256×104.

 

The calculated concentration of rabbit A is 24 mg/mL.

The calculated concentration of rabbit B is 29.9 mg/mL.



3. Result of BCA

Dilute BSA with TE to constitute concentration gradient 0 mg/mL, 0.2 mg/mL, 0.4 mg/mL, 0.8 mg/mL, 1 mg/mL. Do duplicate and make standard curve. Dilute purified IgG 10 times, 20 times, 40 times with TE. Do duplicate (The volume of BCA is 200 μL, the volume of protein is 20 μL), keep at 37℃ for 30 min, read at 568 nm.

 

BSA concentration (mg/mL)

OD568nm

IgG dilution factor

BSA

IgG

Rabbit A IgG

0

0.084

0.081

0.585

0.603

1/40

0.2

0.283

0.281

1.02

1.054

1/20

0.4

0.453

0.469

1.681

1.724

1/10

Rabbit B IgG

0.6

0.605

0.593

0.698

0.72

1/40

0.8

0.748

0.732

1.145

1.184

1/20

1

0.897

0.896

1.818

1.861

1/10



4. Delivery

 

Concentration

Volume

Titer

Components

Amount

Antisera

Negative serum

Rabbit A IgG

24 mg/mL

1 mL

128×104

50% glycerinum2 mM EDTA0.02% P300

4 vials

400 μL

100 μL

Rabbit B IgG

29.9 mg/mL

1 mL

256×104

50% glycerinum2 mM EDTA0.02% P300

6 vials

400 μL

100 μL