One-component TMB Substrate
E-IR-R201
| Size: | Price: | $33 | |
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Application: ELISA
Introduction
TMB is a popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The soluble One-component TMB Substrate are supplied as Ready-to-Use mono-component liquids that require no preparation before use. The product mainly contains 3,3’,5,5’-tetramethylbenzidine and contains no DMF or DMSO.
One-component TMB Substrate yields a blue color (Amax= 370 nm and 652 nm) when reacted with peroxidase in microwell applications. The product changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
One-component TMB Substrate yields a blue color (Amax= 370 nm and 652 nm) when reacted with peroxidase in microwell applications. The product changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Method for use
1. Add 100 μL of the TMB Substrate Solution to each microplate well.
2. Incubate for 5~30 min according to the reaction system.
3. Stop reaction by adding 50 uL stop solution (1M HCl or 1M H2SO4).
4. Measure the absorbance of each well at 450 nm.
2. Incubate for 5~30 min according to the reaction system.
3. Stop reaction by adding 50 uL stop solution (1M HCl or 1M H2SO4).
4. Measure the absorbance of each well at 450 nm.
Storage
2-8℃, 12 months
Shading light.
Shading light.
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