Recombinant Human IGFBP3/IBP3 Protein (His Tag)
PKSH031612
| Size: | Price: | $911 | |
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Lead Time: 7~10 days
Product Details
| Catalog number | PKSH031612 |
| Expression_host | HEK293 Cells |
| Sequence | Gly28-Lys 291 |
| Bio_Activity | 1. Immobilized human IGF2 at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 18 ng/mL. 2. Immobilized human IGF1 at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 24 ng/mL. 3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K. P. et al. (1988) Cancer Research 48:4083. ). The ED50 for this effect is typically 0.02-0.2 μg/mL in the presence of 14 ng/mL human IGFII. |
| Application | Functional ELISA |
| Accession | NP_000589.2 |
| Synonyms | BP-53;IBP3;IGFBP3 |
| Tag | N-His |
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
