Recombinant Human R-Spondin 1/RSPO1 Protein (His Tag)
PKSH031269
| Size: | Price: | $978 | |
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Lead Time: 7~10 days
Product Details
| Catalog number | PKSH031269 |
| Expression_host | HEK293 Cells |
| Sequence | Met 1-Ala 263 |
| Bio_Activity | 1. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind human LIMPII with a linear ranger of 32-800 ng/ml. 2. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 with a linear ranger of 6. 4-800 ng/ml. 3. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a. |
| Application | Functional ELISA |
| Accession | NP_001033722.1 |
| Synonyms | RSPO1;R-spondin1;RP11-566C13.1;CRISTIN3;FLJ40906;RSPO Rspo1;R-spondin;Rspondin;RP23-325M14.2;Roof plate-specific spondin-1 |
| Tag | C-His |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
