Protein Purification&Separation
For most researchers of biochemistry, structural biology and functional proteomics, they will spent a large amount of time and devote plenty of energy in specific protein or other biomolecules separation and purification. The step above is the most basic experiment procedure and the most important one in whole experimentation which is of crucial significance for laboratory result and experimental cost. Only make it efficiently done could we win the match with other peers.
So who is the “clutch player” in this “nonexplosive warfare”? The answer is high-quality Chromatographic Media. Elabscience® offers an extensive range of chromatographic media for most of biomolecules separation and purification. We have collected our best products for all kinds of packing to help you separate and purify your target molecules easily and efficiently. All of these products have been strictly verified by our responsible and talented technicians, thus are guaranteed to be high-quality and can help you to cut cost and get favorable results. View and see how Elabscience® can help you with your biochemical researches.
Gel filtration chromatography, also known as size exclusion chromatography (SEC), is a chromatographic method that separates molecules by their size or molecular weight. SEC can be used for wide ranges of separation and purification of components with large molecular weight differences and low resolution requirement, such as proteins, polysaccharides and other macromolecules.
Cat. No.
Product name
Matrix
Particle size
range
Average particle
size
4% agarose
45-165 µm
90 µm
6% agarose
45-165 µm
90 µm
Cross-linked 4%
agarose
165-300 µm
200 µm
Cross-linked 6%
agarose
165-300 µm
200 µm
Lowly
cross-linked 4% agarose
45-165 µm
90 µm
Lowly
cross-linked 6% agarose
45-165 µm
90 µm
Highly
cross-linked 4% agarose
45-165 µm
90 µm
Highly
cross-linked 6% agarose
45-165 µm
90 µm
Dextran
45-165 µm
100-300 µm
Cross-linked
dextran
45-165 µm
80-300 µm
Affinity chromatography is a powerful chromatography technique of separating and purifying proteins based on the highly specific interaction between antigen and antibody, enzyme and substrate, or receptor and ligand. Such interactions including hydrogen bonding, ionic interaction, disulfide bridges, hydrophobic interaction, etc. Affinity chromatography are widely recognized for their high selectivity, high resolution and high capacity.
Cat. No.
Product name
Matrix
Binding capacity
Highly
cross-linked 4% agarose
16-23 μmol /mL
(media)
Highly
cross-linked 4% agarose
15-25mg
(GST-tagged protein)/mL (medium)
4% agarose
15-25mg
(GST-tagged protein)/mL (media)
Highly
cross-linked 6% agarose
20-30 mg
(His-tagged protein)/mL (medium)
Highly
cross-linked 6% agarose
45 mg
(His-tagged protein)/mL (medium)
Highly
cross-linked 4% agarose
40-50 mg
(His-tagged protein)/mL (medium)
Highly
cross-linked 6% agarose
45 mg
(His-tagged protein)/mL (media)
Highly
cross-linked 4% agarose and cellulose
~30 mg (Human
IgG)/mL (media)
Highly
cross-linked 4% agarose
~25 mg (Human
IgG)/mL (media)
Highly
cross-linked 4% agarose
25-50 mg
(Trypsase)/mL (media)
Highly
cross-linked 6% agarose
0.4-0.5 mM Cl-/mL
(media)
Highly
cross-linked 6% agarose
0.4-0.6 mM Cl-/mL
(media)
Ion exchange chromatography (IEX) is a practical chromatography process that separates ions
and polar molecules based on their respective charged groups. IEX can be used for separation and purification of charged large proteins, small
nucleotides, and amino acids.
Cat. No. |
Product name |
Matrix |
Ionic capacity |
Binding capacity |
Highly cross-linked 6% agarose |
180-300 µmol H+/mL (media) |
100 mg Lysyme /mL (media) |
||
Highly cross-linked 6% agarose |
200-300 µmol H+/mL (media) |
120 mg Lysyme /mL (media) |
||
Highly cross-linked agarose and cellulose |
180-280 µmol H+/mL (media) |
100 mg Lysyme /mL (media) |
||
Highly cross-linked 6% agarose and cellulose |
140-220 µmol H+/mL (media) |
120 mg Lysyme /mL (media) |
||
Highly cross-linked agarose and dextran |
180-250 µmol H+/mL (media) |
>160 mg Lysyme /mL (media) |
||
Highly cross-linked 6% agarose |
90-150 µmol H+/mL (media) |
110 mg Lysyme /mL (media) |
||
Highly cross-linked 6% agarose |
90-160 µmol H+/mL (media) |
95 mg Lysyme /mL (media) |
||
Highly cross-linked 6% agarose |
180-360 µmol Cl-/mL (media) |
90 mg BSA/mL (media) |
||
Highly cross-linked 6% agarose |
200-280 µmol Cl-/mL (media) |
130 mg BSA/mL (media) |
||
Highly cross-linked agarose and cellulose |
180-250 µmol Cl-/mL (media) |
120 mg BSA/mL (media) |
||
Highly cross-linked 6% agarose and cellulose |
160-220 µmol Cl-/mL (media) |
100 mg BSA/mL (media) |
||
Highly cross-linked agarose and dextran |
200-350 µmol Cl-/mL (media) |
>130 mg BSA/mL (media) |
||
Highly cross-linked 6% agarose |
150-300 µmol Cl-/mL (media) |
110 mg BSA/mL (media) |
||
Highly cross-linked agarose and dextran |
460-510 µmol Cl-/mL (media) |
180 mg BSA/mL (media) |
||
Highly cross-linked 6% agarose |
180-260 µmol Cl-/mL (media) |
90 mg BSA/mL (media) |
||
Highly cross-linked 6% agarose |
100-200 µmol Cl-/mL (media) |
80 mg BSA/mL (media) |
||
Highly cross-linked 6% agarose |
90-130 µmol Cl-/mL (media) |
≥75mg BSA/mL (media) |
||
Highly cross-linked 6% agarose |
130-200 µmol Cl-/mL (media) |
≥95mg BSA/mL (media) |
||
Highly cross-linked 6% agarose |
150-250 µmol H+/mL (media) |
≥75 mg BSA/mL (media) |
||
Highly cross-linked 6% agarose |
120-180 µmol H+/mL (media) |
≥60 mg BSA/mL (media) |
Hydrophobic interaction chromatography (HIC) is a useful method for purification and separation of biomolecules based on their surface hydrophobicity. HIC can be applied in the separation and purification of hydrophobic proteins such as aromatic and aliphatic compounds. In HIC, the matrix material is lightly substituted with hydrophobic groups, such as methyl, ethyl, propyl, octyl, or phenyl groups.
Cat. No. |
Product name |
Matrix |
Ligand density |
Binding capacity |
Highly cross-linked 6% agarose |
40-50 µmol/mL |
40 mg (IgG)/mL (media) |
||
Highly cross-linked 6% agarose |
15-20 µmol/mL |
15 mg (IgG)/mL (media) |
||
Highly cross-linked 6% agarose |
25-30 µmol/mL |
30 mg (IgG)/mL (media) |
||
Cross-linked 6% agarose |
25-30 µmol/mL |
25 mg (IgG)/mL (media) |
||
Highly cross-linked 6% agarose |
10 µmol/mL |
10-20 mg (Lysyme)/mL (media) |
||
Highly cross-linked 4% agarose |
~5 µmol/mL |
~10 mg (Lysyme)/mL (media) |