TUNEL Assay Kit
The TUNEL series of apoptosis kits developed by Elabscience®, including the One-step TUNEL In Situ Apoptosis Kit, One-step TUNEL Flow Cytometry Apoptosis Kit, and TUNEL In Situ Apoptosis Kit (HRP-DAB Method), can be used for apoptosis detection of tissue samples (paraffin-embedded, frozen section) and cells samples (cell smears, cell crawling films, suspension cells, adherent cells).Our TUNEL assay kits have high sensitivity, quick and easy operation, which can better assist in apoptosis research on diseases related to cell function and R&D of related drugs.
Detection Principle
An important feature of the late stage of apoptosis is DNA fragmentation, and the exposed 3'-OH of the fragmented DNA can be catalyzed by terminal deoxynucleotidyl transferase (TdT) to bind to fluorochrome-labeled dUTP, which can be visualized by fluorescence microscopy or flow cytometry. This method is also known as One-step method (higher detection sensitivity, more intuitive results, and easier experimental results to be published in SCI articles).
If the exposed 3'-OH is added with biotin-labeled dUTP (Biotin-dUTP) under the catalysis of TdT, and then combined with HRP-labeled Streptavidin (Streptavidin-HRP), it is finally displayed by DAB color development under the catalysis of HRP, apoptotic cells can be detected by ordinary optical microscope.
One-step TUNEL In Situ Apoptosis Kit
Elabscience® One-step TUNEL In Situ Apoptosis Kits are suitable for in situ apoptosis detection of tissue samples (paraffin-embedded, frozen section) and cell samples (cell smear, slide film), and the detection results can be directly observed by fluorescence microscope. Compared with the DAB colorimetric method, the experimental results of fluorescent TUNEL are fluorescent pictures, which are more intuitive and easy to be published in SCI articles.
- Multiple choices: A variety of fluorescent dyes (FITC/ Elab Fluor® 488/ Elab Fluor® 594/ Elab Fluor® 647/ Elab Fluor® 555) kits are available
- Easy to operate: One-step dyeing, saving time and effort
- Safe and non-toxic: No dimethyl arsenate, non-toxic and harmless to people and the environment
- Cost-effective: The key components are self-developed with more stable quality, and more cost-effective price
- Wide range of applications: Suitable for a variety of tissue sample (paraffin section, frozen section) and cells sample (cell slide)
- View Elabscience® One-step TUNEL In Situ Apoptosis Kit
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ElabscienceTraditional dewaxing methods
Green fluorescence
(Illumination value: 10, exposure time: 100 ms)
| Product Name | Dye | Excitation/ emission wavelength |
|---|---|---|
| One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | FITC, DAPI | FITC: 495/525nm DAPI: 350/470nm |
| One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488) | Elab Fluor® 488, DAPI | Elab Fluor® 488: 495/519nm DAPI: 350/470nm |
| One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594) | Elab Fluor® 594, DAPI | Elab Fluor® 594: 590/617nm DAPI: 350/470nm |
| One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 647) | Elab Fluor® 647, DAPI | Elab Fluor® 647: 650/665nm DAPI: 350/470nm |
| One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 555) | Elab Fluor® 555, DAPI | Elab Fluor® 555: 555/565nm DAPI: 350/470nm |
One-step TUNEL Flow Cytometry Apoptosis Kit
Elabscience® One-step TUNEL Flow Cytometry Apoptosis Kit is a highly sensitive and quick and easy detection product for apoptosis. This kit is suitable for apoptosis detection of suspension cells and adherent cells, and the detection results can be analyzed by flow cytometry.
- Better detection scheme: Optimized fluorescent labeling solution, more suitable for flow detection scheme
- Multiple choices: FITC/ Elab Fluor® 488/ Elab Fluor® 594/ Elab Fluor® Violet 450/ Elab Fluor® 647/ Elab Fluor® 555, a variety of fluorescence channels are available
- Easy to operate: One-step dyeing, saving time and effort
- Cost-effective: The kit contains fixed permeabilization fluid, no need to purchase additional reagents
- Wide range of applications: Suitable for a variety of suspension cells and adherent cultured cells
- View Elabscience® One-step TUNEL Flow Cytometry Apoptosis Kit
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Click to enlarge the picture
Biological control: Jurkat cells cultured without camptothecin, labeled with working solution, and TdT enzyme added
Negative control: Jurkat cells cultured with 5μM camptothecin for 4h, labeled with working solution, without TdT enzyme
Experimental group: Jurkat cells cultured with 5 μM camptothecin for 4 h, labeled with working solution, and TdT enzyme added
| Product Name | Dye | Excitation/ emission wavelength |
|---|---|---|
| One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC) | FITC | 495/525nm |
| One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) | Elab Fluor® 488 | 495/519nm |
| One-step TUNEL Flow Cytometry Apoptosis Kit (Red, Elab Fluor® 594) | Elab Fluor® 594 | 590/617nm |
| One-step TUNEL Flow Cytometry Apoptosis Kit (Blue, Elab Fluor® Violet 450) | Elab Fluor® Violet 450 | 410/450nm |
| One-step TUNEL Flow Cytometry Apoptosis Kit (Red, Elab Fluor® 647) | Elab Fluor® 647 | 650/665nm |
| One-step TUNEL Flow Cytometry Apoptosis Kit (Red, Elab Fluor® 555) | Elab Fluor® 555 | 555/565nm |
TUNEL In Situ Apoptosis Kit (HRP-DAB Method)
Elabscience® TUNEL In Situ Apoptosis Kit (HRP-DAB Method) is a product with high sensitivity and can detect apoptosis quickly and easily. This kit is suitable for tissue samples (paraffin sections, frozen sections) and cell samples (cell smears, cell slides). After biotin labeling and subsequent DAB color development, apoptosis can be observed with ordinary optical microscope.
- High sensitivity: Very low background staining, strong positive staining and apoptosis can be detected at the single-cell level
- Good specificity:It is generally easier to label apoptotic cells than necrotic cells in TUNEL assays
- Quick and easy: It only takes about 2-3 h to complete the staining
- Safe and non-toxic: No dimethyl arsenate, non-toxic and harmless to people and the environment
- Wide range of applications: Suitable for a variety of tissue sample (paraffin section, frozen section) and cells sample (cell slide)
Click to view TUNEL In Situ Apoptosis Kit (HRP-DAB Method)
Experiment Guide
☞ How to choose an appropriate TUNEL assay kit?
☞ View One-step TUNEL (Cell Slide) Operation Guide (Video)
☞ View One-step TUNEL (Paraffin Section) Operation Guide (Video)
☞ View One-step TUNEL (Frozen Section) Operation Guide (Video)
☞ View One-step TUNEL (Flow Cytometry) Operation Guide（video）
☞ View TUNEL In Situ Apoptosis Kit (HRP-DAB Method) Operation Guide (Video)
