293T [HEK-293T] Cell Line
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Datasheet
Origin and General Characteristics
| Cell Name | 293T [HEK-293T] Cell Line |
| Organism | Homo sapiens, Human |
| Age | Fetus |
| Tissue | Embryonic kidney |
| Morphology | Epithelial |
| Growth Properties | Adherent |
| Descriptions | This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses. It has been widely used for retroviral production, gene expression and protein production. |
| Biosafety Level | 2 |
Culture Conditions and Handling
| Complete Growth Medium | DMEM +10% FBS +1% P/S |
| Subculturing | Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. |
| Subcultivation Ratio | 1:3-1:6 |
| Medium Renewal | Every 2 to 3 days |
| Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃ |