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Sensitivity | 0.94 ng/mL |
Detection Range | 1.56-100 ng/mL |
Sample Volume | 50 μL |
Total Assay Time | 2 h 30 min |
Reacitivity | Universal |
Specificity | This kit recognizes Universal 3-NT in samples.No significant cross-reactivity or interference between Universal 3-NT and analogues was observed |
Recovery | 80%-120% |
Sample Type | Serum, plasma and other biological fluids |
Detection Method | Colorimetric method, ELISA, Competitive |
Assay Type | Competitive-ELISA |
Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
Storage | 2-8℃ |
Expiration Date | 12 months |
Research Area | Signal Transduction |
Other Clones
1 Results
Other Formats
1 Results
- Ratiometric SERS imaging for indication of peroxynitrite fluctuations in diabetic wound healing process
IF:15.100
Journal:CHEMICAL ENGINEERING JOURNAL(2023)
DOI:10.1016/j.cej.2023.144024Reactivity:Mouse
Sample Type:Blood
- Nitrosative and Oxidative Stress, Reduced Antioxidant Capacity, and Fiber Type Switch in Iron-Deficient COPD Patients: Analysis of Muscle and Systemic Compartments
IF:5.900
Journal:Nutrients(2023)
DOI:10.3390/nu15061454Reactivity:Human
Sample Type:serum
- Association of hydroxytyrosol enriched olive oil with vascular function in chronic coronary disease
IF:5.500
Journal:EUROPEAN JOURNAL OF CLINICAL INVESTIGATION(2023)
DOI:10.1111/eci.13983Reactivity:Human
Sample Type:serum
- P66Shc is increased in peripheral blood mononuclear cells of the patients with obstructive sleep apnea
IF:3.600
Journal:International Journal of Medical Sciences(2023)
DOI:10.7150/ijms.80343Reactivity:Human
Sample Type:plasma
- Inflammatory signal transduction pathways induced by prilocaine toxicity in cultured ARPE-19 cells
IF:3.600
Journal:JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY(2023)
DOI:10.1002/jbt.23491Reactivity:Human
- Neuroprotection by trans-resveratrol in rats with N-methyl-D-aspartate (NMDA)–induced retinal injury: Insights into the role of adenosine A1 receptors
IF:2.900
Journal:NEUROSCIENCE RESEARCH(2023)
DOI:10.1016/j.neures.2023.02.004Reactivity:Rat
Sample Type:retinal
- Ambient fine particulate matter exposures and oxidative protein damage in early pregnant women
IF:9.988
Journal:ENVIRONMENTAL POLLUTION(2022)
DOI:10.1016/j.envpol.2022.120604Reactivity:Human
Sample Type:serum
- Attenuated effect of zinc gluconate on oxidative stress, inflammation, and angiogenic imbalance in pre-eclampsia rats
IF:6.780
Journal:LIFE SCIENCES(2022)
DOI:10.1016/j.lfs.2022.121055Reactivity:Rat
Sample Type:serum, placental
- Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species
IF:5.581
Journal:Metabolites(2022)
DOI:10.3390/metabo13010049Reactivity:Rat
Sample Type:serum
- Protection against Doxorubicin-Induced Cardiotoxicity through Modulating iNOS/ARG 2 Balance by Electroacupuncture at PC6
IF:6.543
Journal:Oxidative Medicine and Cellular Longevity(2021)
DOI:10.1155/2021/6628957Reactivity:Mouse
Sample Type:Tissue homogenate
Q1:Why is it necessary to add a protease inhibitor in tissue sample preparation during an Elisa experiment? Will it affect the detection significantly if there is no protease inhibitor?
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
Q2:My sample volume is small. Can I reduce some reagents proportionally?
No, the detection system of our kits requires strict adherence to the specified sample volume to ensure accurate detection. If the sample volume is insufficient, consider diluting appropriately, but first conduct a pre-experiment to confirm the suitable dilution factor.
Q3:I need to measure corticosterone and testosterone in hair samples. Are there any suggested sample extraction methods?
Hair sample preparation method: Wash hair samples with methanol by adding 5 mL of HPLC-grade methanol to each sample, rotating for 3 minutes, then decanting excess methanol and rinsing hair twice. After washing, place the hair samples on aluminum foil, dry for 3 days in a protective cap. Weigh the dried hair samples and transfer them to 2ml polypropylene tubes containing stainless steel grinding beads. Place the tubes containing hair and beads in a bead beater, grind each sample for 2 minutes to produce powder. After grinding, add 1.5 mL of methanol to the tubes containing hair powder. Rotate samples slowly for 24 hours to extract steroids. Centrifuge at 10000 g for 4 minutes, transfer 0.6 mL of methanol supernatant containing steroids to new 1.5 mL microcentrifuge tubes. Dry the samples in a protective hood for 2-3 days to evaporate the methanol. Dilute the dried extract with 0.4 mL dilution buffer from the kit for detection.
Q4:Do your ELISA kits have the enzyme that catalyzes the substrate reaction is not HRP? My sample is red blood cell lysate, and HRP will interfere with hemoglobin. Or is there a way to remove Hb?
The detection antibodies in our ELISA kits are all HRP-based. Currently, there are no kits available for testing red blood cell lysate. For small amounts of hemoglobin, consider increasing the number of washing steps by 1-2 during the first plate washing step to reduce interference. However, besides hemoglobin, substances such as endogenous HRP enzymes released during the process of red blood cell lysis can also catalyze the substrate, so we still do not recommend testing with such samples.
Q5:Do you have ELISA kits for plant or microbial samples?
Currently, our ELISA kits are only suitable for animal samples, and we do not have kits for testing plant/microbial samples.
Q6:Do you have a suggested preparation method for vaginal discharge samples?
After the collection with the swab, elute with 500μl PBS, centrifuge at 5000×g for 10 minutes (if the sample remains turbid, try centrifuging at 10000×g), then collect the supernatant for testing.
Q7:Can ELISA kits be used on automated analyzers?
Our ELISA kits have not been validated for compatibility with automated analyzers. The provided instructions are for manual operation. Customers may validate them on their own if needed.
Q8:Can a shaker or a water bath be used during the incubation stage in ELISA experiments?
To ensure temperature fluctuations during incubation stay within 1°C, it's recommended to use an incubator. If an incubator is not available, a water bath may be used, but precautions should be taken to avoid contaminating the plate with water. Using a shaker, especially one with vibration, for incubation is not recommended.