7-AAD Viability Staining Solution[162] manufacturer - Elabscience

×

Added to cart successfully !

7-AAD Viability Staining Solution[162]

Size:  
Qty:
- +
Price:   $20
Cat Number:  E-CK-A162
Lead Time:   Order now, ship in 3 days
Add to cart

Introduction

Elabscience® 7-AAD Viability Staining Solution is developed to identify apoptotic and necrotic cells.

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using 7-AAD and Annexin V.



Components


Cat.

Products

50 Tests

100 Tests

200 Tests

500 Tests

Storage

E-CK-A162

7-AAD Viability Staining Solution(100 μg/mL)

250 μL

500 μL

1 mL

1.25 mLx2

-20°C

Manual

 One Copy





Jurkat cells were treated with 5 μM Camptothecin and detected by this reagent and Annexin V-APC.


Jurkat cells were treated with 5 μM Camptothecin (Right) or not (Left) for 4 h. Annexin V-APC single-positive cells were early apoptotic cells, Annexin V-APC and 7-AAD double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were nude nuclear cells.


Staining Procedure

1. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.

Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware!

2. Split the cell suspension into tubes, 1-5 × 105 cells for each, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer[E-CK-A151] to resuspend the cells.

3. Add 5 µl of Annexin V-APC[E-CK-A117] and 5 µl of 7-AAD Viability Staining Solution to each tube.

4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.

5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.


Storage

Store at -20°C for one year in dark. 7-AAD Viability Staining Solution should be spilt into small tubes.


Cautions

1. For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.

2. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.

3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.

4. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

If you have any question about the products, please contact us by clicking the message button near the home button of your phone, we will reply you in 12 hours.