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Annexin V-FITC / PI Apoptosis Detection Kit[211]

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Qty:
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Price:   $75
Cat Number:  E-CK-A211
Lead Time:   7~10 days

Applications:  Apoptosis

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Introduction

Elabscience® Annexin V-FITC/PI Cell Apoptosis Detection kit is developed to identify apoptotic and necrotic cells.

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-FITC, the FITC-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.

Propidium Iodide (PI) is a common DNA dye that is not permeable to cell membrane. Once binding to DNA, the flurescence of PI increases by nearly 20 fold. Due to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.



Components

Cat.

Products

20 Tests

50 Tests

100 Tests

200 Tests

Storage

E-CK-A111

Annexin V-FITC Reagent

100 μL

250 μL

500 μL

1 mL

2~8°C

E-CK-A151

Annexin V Binding Buffer (10 ×)

1.4 mL×2

5.5 mL

11 mL

11 mL×2

2~8°C

E-CK-A161

Propidium Iodide (PI) Staining Solution

100 μL

250 μL

500 μL

1 mL

2~8°C

Manual

 One Copy



Jurkat cells were treated with 5 μM Camptothecin and detected with this kit.


Jurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-FITC single-positive cells were early apoptotic cells, Annexin V-FITC and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.


Instructions

The Annexin V Binding Buffer (10 ×)[E-CK-A151] is a 10 × concentrated solution. Dilute with DI water to 1 × working solution before use.

For example: Take 1 mL Annexin V Binding Buffer (10 ×), dilute with DI water to 10 mL.


Storage

Store at 2~8°C. Annexin V-FITC and Propidium Iodide (PI)  Staining Solution should be stored in dark.


Cautions

1. For maximal assay performance, this kit should be used within 12 months. Avoid freeze / thaw cycles.

2. For FCM analysis, please set untreated cells stained with both Annexin V-FITC and PI as negative control. As for compensation controls, please use drug-treated cells stained with either Annexin V-FITC or PI.

3. Annexin V-FITC can be detected in FITC channel while PerCP/Cy5.5 channel is preferred to PE channel for PI detection due to the large compensation needed between FITC and PE channels.

4. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.

5. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.

6. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

If you have any question about the product, please feel free to contact us or leave a message