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APC Anti-Human CD16 Antibody[CB16] E-AB-F1005E

Host: Mouse

Applications: FCM

Isotype: Mouse IgG1, κ

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Human pheripheral blood cells are stained with APC Anti-Human CD16 Antibody(filled gray histogram). Unstained pheripheral blood cells (blank black histogram) are used as control.

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Price:   $55
Lead Time:   7~10 days
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Antigen Details

Alternate Names Low affinity immunoglobulin gamma Fc region receptor III-A,FCGR3A,CD16a antigen,Fc-gamma RIII-alpha,Fc-gamma RIII,Fc-gamma RIIIa,FcRIII,FcRIIIa,FcR-10,IgG Fc receptor III-2,CD16a
Swissprot P08637
References
1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Product Details

Form Liquid
Clone No. CB16(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control APC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792E]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer PBS with 0.05% Proclin300, 1% BSA
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.

Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC

Background

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F1005C FITC Anti-Human CD16 Antibody[CB16] E-AB-F1005C 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1005D PE Anti-Human CD16 Antibody[CB16] E-AB-F1005D 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1005E APC Anti-Human CD16 Antibody[CB16] E-AB-F1005E 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1005H PE/Cyanine7 Anti-Human CD16 Antibody[CB16] E-AB-F1005H 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1005J PerCP/Cyanine5.5 Anti-Human CD16 Antibody[CB16] E-AB-F1005J 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1005L AF488 Anti-Human CD16 Antibody[CB16] E-AB-F1005L 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1005M AF647 Anti-Human CD16 Antibody[CB16] E-AB-F1005M 20 Tests, 50 Tests, 100 Tests, 200 Tests
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