APC Anti-Human CD16 Antibody[CB16] E-AB-F1005E
Host: Mouse
Applications: FCM
Isotype: Mouse IgG1, κ
Filter:
FCM
Human pheripheral blood cells are stained with APC Anti-Human CD16 Antibody(filled gray histogram). Unstained pheripheral blood cells (blank black histogram) are used as control.
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| Price: | $55 |
Datasheet
Antigen Details
| Alternate Names | Low affinity immunoglobulin gamma Fc region receptor III-A,FCGR3A,CD16a antigen,Fc-gamma RIII-alpha,Fc-gamma RIII,Fc-gamma RIIIa,FcRIII,FcRIIIa,FcR-10,IgG Fc receptor III-2,CD16a |
| Swissprot | P08637 |
| References |
1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572. 3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381. |
Product Details
| Form | Liquid |
| Clone No. | CB16(See Other Available Formats) |
| Host | Mouse |
| Isotype | Mouse IgG1, κ |
| Isotype Control | APC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792E] |
| Reactivity | Human |
| Application | FCM (See the Application Description) |
| Storage Buffer | PBS with 0.05% Proclin300, 1% BSA |
| Recommended Use | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
| Shipping | Biological ice pack at 4℃ |
| Stability & Storage | Keep as concentrated solution. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
| Conjugation | APC |
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Background
| CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). |
Protocols & Troubleshootings
| Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
| Troubleshooting |
Alternative Formats
| Cat.No. | Product Name | Sizes |
| E-AB-F1005C | FITC Anti-Human CD16 Antibody[CB16] E-AB-F1005C | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1005D | PE Anti-Human CD16 Antibody[CB16] E-AB-F1005D | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1005E | APC Anti-Human CD16 Antibody[CB16] E-AB-F1005E | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1005H | PE/Cyanine7 Anti-Human CD16 Antibody[CB16] E-AB-F1005H | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1005J | PerCP/Cyanine5.5 Anti-Human CD16 Antibody[CB16] E-AB-F1005J | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1005L | AF488 Anti-Human CD16 Antibody[CB16] E-AB-F1005L | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1005M | AF647 Anti-Human CD16 Antibody[CB16] E-AB-F1005M | 20 Tests, 50 Tests, 100 Tests, 200 Tests |