BV2 Cell Line
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Datasheet
Origin and General Characteristics
| Cell Name | BV2 Cell Line |
| Organism | Mus musculus, Mouse |
| Tissue | Brain |
| Morphology | Morphology microglial |
| Growth Properties | Mixed, Adherent And Suspension |
| Biosafety Level | 1 |
Culture Conditions and Handling
| Complete Growth Medium | MEM +10% FBS +1% P/S |
| Subculturing | Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | Every 2 to 3 days |
| Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃ |