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GABRG1 Polyclonal Antibody (E-AB-64959)

AllSizePriceQty
200μL $ 530.00
120μL $ 320.00
60μL $ 200.00
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For research use only. Order now, ship in 3 days

Verified Samples Verified Samples in WB: U87-MG, Mouse brain
Dilution WB 1:500-1:2000
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB
ClonalityPolyclonal
ImmunogenRecombinant fusion protein of human GABRG1 (NP_775807.2).
AbbreGABRG1
SynonymsGABRG1
Swissprot
Calculated MW53 kDa
Observed MW 54 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular LocalizationCell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse.
Concentration1 mg/mL
BufferPhosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification MethodAffinity purification
Research AreasNeuroscience
ConjugationUnconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
ShippingThe product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
backgroundThe protein encoded by this gene belongs to the ligand-gated ionic channel family. It is an integral membrane protein and plays an important role in inhibiting neurotransmission by binding to the benzodiazepine receptor and opening an integral chloride channel. This gene is clustered with three other family members on chromosome 4.
Cat.No. Product Name Sizes
E-AB-1003 Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1194 HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

1 Results

    Other Formats

    1 Results

    Unconjugated