Toll-free:1-888-852-8623

All categories

  • All categories
  • Flow Cytometry Antibodies
  • ELISA Kits
  • MACS Cell Isolation
  • Antibodies and Reagents
  • Cell Health Detection
  • Metabolism Assays
  • Immunoassays
  • Cell Identification
  • Proteins and Peptides
  • Cell Culture
Please enter the item number/product keyword!
Keyword cannot be empty !
INSERT SYMBOLS:
  • α
  • β
  • γ
  • δ
  • ε
  • ζ
  • η
  • θ
  • κ
  • μ
  • ω
  • σ
  • τ
  • λ
  • ⅩⅢ
  • ⅩⅢ
  • ⅩⅣ
  • ⅩⅤ
  • ⅩⅦ
  • ⅩⅧ
  • UP ↑

GOLGB1 Polyclonal Antibody (E-AB-92244)

  • +1
AllSizePriceQty
200μL $ 530.00
120μL $ 320.00
60μL $ 200.00
Add to cart

For research use only.

Verified Samples Verified Samples in WB: HeLa
Verified Samples in IHC: Human colon carcinoma, Human esophageal cancer
Verified Samples in IF: U2OS
Dilution WB 1:500-1:2000,  IHC 1:50-1:200,  IF 1:50-1:200
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB,  IHC,  IF
ClonalityPolyclonal
ImmunogenRecombinant fusion protein of human GOLGB1
Synonyms GCP372,   GOLIM1,  GCP
Swissprot
Calculated MW376 kDa
Observed MW 372 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular LocalizationCis-Golgi network, cytosol, endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Golgi stack.
Concentration1 mg/mL
BufferPhosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification MethodAffinity purification
Research AreasTags,  Cell Markers
ConjugationUnconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
ShippingThe product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
backgroundMay participate in forming intercisternal cross-bridges of the Golgi complex.
Other Clones

1 Results

    Other Formats

    1 Results

    Unconjugated