HEL Cell Line-Laboratory Biological Research Reagents Manufacturer for Life Science - Elabscience

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HEL Cell Line

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Cat Number:  EP-CL-0280
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Origin and General Characteristics

Cell Name HEL Cell Line
Organism Homo sapiens, Human
Tissue Peripheral blood
Morphology Rounded
Growth Properties Mixed, Adherent And Suspension
Biosafety Level 1

Culture Conditions and Handling

Complete Growth Medium RPMI-1640 +10% FBS +1% P/S
(HEL Cell Line complete medium (EP-ML-0280) is recommended.)
Subculturing Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio 1:2-1:4
Medium Renewal Every 2 to 3 days
Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

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Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

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