HEL Cell Line
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Datasheet
Origin and General Characteristics
| Cell Name | HEL Cell Line |
| Organism | Homo sapiens, Human |
| Tissue | Peripheral blood |
| Morphology | Rounded |
| Growth Properties | Mixed, Adherent And Suspension |
| Biosafety Level | 1 |
Culture Conditions and Handling
| Complete Growth Medium | RPMI-1640 +10% FBS +1% P/S (HEL Cell Line complete medium (EP-ML-0280) is recommended.) |
| Subculturing | Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | Every 2 to 3 days |
| Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃ |
Related Products
| EP-CM-P0001 | RPMI-1640, powder |
| EP-CM-L0301 | RPMI-1640 |
| EP-CM-L0048 | Penicillin-Streptomycin Solution (100×) |
| EP-CM-L0006 | Phosphate Buffer (PBS) |
| EP-CM-L0060 | 200mM L-Alanyl-L-Glutamine Solution (100×) |
Technical Resources
Recommendations for handling of cryopreserved cells
Protocol for Thawing Frozen Cells
