High Sensitivity Rat IL-10 (Interleukin 10) ELISA Kit (E-HSEL-R0005)
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New series of Elabscience® High Sensitivity (HS) ELISA kits have been iteratively upgraded through R&D process to increase the sensitivity of the traditional ELISA kits by 2-10 times, and can detect the protein concentration of fg level. High Sensitivity (HS) ELISA Kit focuses on the detection of cytokines, and this series can effectively solve the problem of low detection rate when detecting low concentration samples such as serum and plasma.
Sensitivity | 1.22 pg/mL |
Detection Range | 3.13-200 pg/mL |
Sample Volume | 100 μL |
Total Assay Time | 3 h 30 min |
Reacitivity | Rat |
Specificity | This kit recognizes Rat IL-10 in samples.No significant cross-reactivity or interference between Rat IL-10 and analogues was observed |
Recovery | 80%-120% |
Sample Type | Serum, plasma and other biological fluids |
Detection Method | Colorimetric method, ELISA, Sandwich |
Assay Type | Sandwich-ELISA |
Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
Storage | 2-8℃ |
Expiration Date | 12 months |
Uniport ID | P29456 |
Research Area | Cancer, Immunology |
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1 Results
- Three Birds, One Stone: An Osteo-Microenvironment Stage-Regulative Scaffold for Bone Defect Repair through Modulating Early Osteo-Immunomodulation, Middle Neovascularization, and Later Osteogenesis
IF:15.100
Journal:Advanced Science(2023)
DOI:10.1002/advs.202306428Reactivity:Rat
Sample Type:cranial bone
- Natural Targeting Potent ROS-Eliminating Tungsten-Based Polyoxometalate Nanodots for Efficient Treatment of Pulmonary Hypertension
IF:10.000
Journal:Advanced Healthcare Materials(2023)
DOI:10.1002/adhm.202300252Reactivity:Rat
Sample Type:lung
- Dual cross-linked gellan gum/gelatin-based multifunctional nanocomposite hydrogel scaffold for full-thickness wound healing
IF:8.200
Journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES(2023)
DOI:10.1016/j.ijbiomac.2023.126349Reactivity:Rat
Sample Type:serum
- Quince extract resists atherosclerosis in rats by down-regulating the EGFR/PI3K/Akt/GSK-3β pathway
IF:7.500
Journal:BIOMEDICINE & PHARMACOTHERAPY(2023)
DOI:10.1016/j.biopha.2023.114330Reactivity:Rat
Sample Type:serum
- Extracellular vesicles derived from human ESC–MSCs target macrophage and promote anti-inflammation process, angiogenesis, and functional recovery in ACS-induced severe skeletal muscle injury
IF:7.500
Journal:Stem Cell Research & Therapy(2023)
DOI:10.1186/s13287-023-03530-1Reactivity:Rat
Sample Type:serum
- Ginger mitigated the health risks associated with arsenic-contamination of rats feed via inflammatory and apoptosis regulation
IF:6.800
Journal:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY(2023)
DOI:10.1016/j.ecoenv.2023.115768Reactivity:Rat
Sample Type:liver tissue
- Protective effects of harmine on Monosodium Iodoacetate-induced Osteoarthritis in rats: In vitro and in vivo studies
IF:6.000
Journal:Arabian Journal of Chemistry(2023)
DOI:10.1016/j.arabjc.2023.104748Reactivity:Rat
Sample Type:serum
- Nifuroxazide repurposing for protection from diabetes-induced retinal injury in rats: Implication of oxidative stress and JAK/STAT3 axis
IF:6.000
Journal:BIOFACTORS(2023)
DOI:10.1002/biof.2011Reactivity:Rat
Sample Type:retinal tissues
- Betulinic acid protects against cardiotoxicity of the organophosphorus pesticide chlorpyrifos by suppressing oxidative stress, inflammation, and apoptosis in rats
IF:5.800
Journal:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH(2023)
DOI:10.1007/s11356-023-25917-6Reactivity:Rat
Sample Type:heart
- Protective effect of arbutin against cyclophosphamide-induced oxidative stress, inflammation, and hepatotoxicity via Nrf2/HO-1 pathway in rats
IF:5.800
Journal:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH(2023)
DOI:10.1007/s11356-023-27354-xReactivity:Rat
Sample Type:liver
Q1:Why is it necessary to add a protease inhibitor in tissue sample preparation during an Elisa experiment? Will it affect the detection significantly if there is no protease inhibitor?
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
Q2:My sample volume is small. Can I reduce some reagents proportionally?
No, the detection system of our kits requires strict adherence to the specified sample volume to ensure accurate detection. If the sample volume is insufficient, consider diluting appropriately, but first conduct a pre-experiment to confirm the suitable dilution factor.
Q3:I need to measure corticosterone and testosterone in hair samples. Are there any suggested sample extraction methods?
Hair sample preparation method: Wash hair samples with methanol by adding 5 mL of HPLC-grade methanol to each sample, rotating for 3 minutes, then decanting excess methanol and rinsing hair twice. After washing, place the hair samples on aluminum foil, dry for 3 days in a protective cap. Weigh the dried hair samples and transfer them to 2ml polypropylene tubes containing stainless steel grinding beads. Place the tubes containing hair and beads in a bead beater, grind each sample for 2 minutes to produce powder. After grinding, add 1.5 mL of methanol to the tubes containing hair powder. Rotate samples slowly for 24 hours to extract steroids. Centrifuge at 10000 g for 4 minutes, transfer 0.6 mL of methanol supernatant containing steroids to new 1.5 mL microcentrifuge tubes. Dry the samples in a protective hood for 2-3 days to evaporate the methanol. Dilute the dried extract with 0.4 mL dilution buffer from the kit for detection.
Q4:Do your ELISA kits have the enzyme that catalyzes the substrate reaction is not HRP? My sample is red blood cell lysate, and HRP will interfere with hemoglobin. Or is there a way to remove Hb?
The detection antibodies in our ELISA kits are all HRP-based. Currently, there are no kits available for testing red blood cell lysate. For small amounts of hemoglobin, consider increasing the number of washing steps by 1-2 during the first plate washing step to reduce interference. However, besides hemoglobin, substances such as endogenous HRP enzymes released during the process of red blood cell lysis can also catalyze the substrate, so we still do not recommend testing with such samples.
Q5:Do you have ELISA kits for plant or microbial samples?
Currently, our ELISA kits are only suitable for animal samples, and we do not have kits for testing plant/microbial samples.
Q6:Do you have a suggested preparation method for vaginal discharge samples?
After the collection with the swab, elute with 500μl PBS, centrifuge at 5000×g for 10 minutes (if the sample remains turbid, try centrifuging at 10000×g), then collect the supernatant for testing.
Q7:Can ELISA kits be used on automated analyzers?
Our ELISA kits have not been validated for compatibility with automated analyzers. The provided instructions are for manual operation. Customers may validate them on their own if needed.
Q8:Can a shaker or a water bath be used during the incubation stage in ELISA experiments?
To ensure temperature fluctuations during incubation stay within 1°C, it's recommended to use an incubator. If an incubator is not available, a water bath may be used, but precautions should be taken to avoid contaminating the plate with water. Using a shaker, especially one with vibration, for incubation is not recommended.