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Human IL-6(Interleukin 6) ELISA Kit

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Price:   $495
Cat Number:  E-EL-H0102
Lead Time:   7~10 days

Reactivity:  Human

Detection Range:  7.81~500 pg/mL

Sensitivity:  4.69 pg/mL

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Intended use

This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-6. You can calculate the concentration of Human IL-6 in the samples by comparing the OD of the samples to the standard curve.

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Reactivity Human
Detection Method Colormetric
Detection Range 7.81—500 pg/mL
Sensitivity 4.69 pg/mL
Sample Volume Required Per Well 100μL
Sample Type serum, plasma and other biological fluids

Specificity

This kit recognizes Human IL-6 in samples. No significant cross-reactivity or interference between Human IL-6 and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration
(pg/mL)
O.D Average Corrected
500 2.463
2.487
2.475 2.407
250 1.612
1.636
1.624 1.556
125 1.012
0.978
0.995 0.927
62.5 0.513
0.533
0.523 0.455
31.25 0.257
0.245
0.251 0.183
15.63 0.174
0.17
0.172 0.104
7.81 0.119
0.123
0.121 0.053
0 0.067
0.069
0.068 --

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-6 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-6 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 25.30 67.60 210.10 24.20 66.50 197.40
Standard deviation 1.70 2.80 7.80 1.60 3.40 6.70
C V (%) 6.72 4.14 3.71 6.61 5.11 3.39

Recovery

The recovery of Human IL-6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum (n=5) 97-110 102
EDTA plasma (n=5) 92-108 99
Cell culture media (n=5) 95-109 101

Linearity

Samples were spiked with high concentrations of Human IL-6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 100-111 89-103 88-101
Average (%) 105 97 93
1:4 Range (%) 97-108 83-96 94-109
Average (%) 102 90 100
1:8 Range (%) 94-109 83-97 95-110
Average (%) 101 89 102
1:16 Range (%) 99-112 84-96 93-105
Average (%) 106 88 99

Kit components & Storage

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4℃(shading light)
Stop Solution 1 vial, 10 mL 4℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

  • Microplate reader with 450 nm wavelength filter
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37℃
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

Assay Procedure

1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

2.Remove the liquid.

3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

6.Add 50 μL Stop Solution.

7.Read at 450 nm immediately. Calculation of results.

Citations

  1. Publication: Carpino G, Del Ben M, Pastori D, et al. Increased Liver Localization of Lipopolysaccharides in Human and Experimental NAFLD[J]. Hepatology, 2020.
    Sample Type: Serum
  2. Publication: Chen W H, Luo G F, Lei Q, et al. Overcoming The Heat Endurance Of Tumor Cells By Interfering The Anaerobic Glycolysis Metabolism For Improved Photothermal Therapy[J]. Acs Nano, 2017, 11(2): 1419-1431.
    Sample Type: Cell culture supernatant
  3. Publication: Liu J, Lu C Y, Zhou H, et al. Flexible Gold Electrode Array For Multiplexed Immunoelectrochemical Measurement Of Three Protein Biomarkers For Prostate Cancer[J]. ACS Applied Materials & Interfaces, 2014, 6(22): 20137-20143.
  4. Publication: Singh G, Nayal A, Malhotra S, et al. Dual functionalized chitosan based composite hydrogel for haemostatic efficacy and adhesive property[J]. Carbohydrate Polymers, 2020, 247: 116757.
    Sample Type: Cell culture supernatant
  5. Publication: Luo F, Guo H, Yu H, et al. PM2. 5 organic extract mediates inflammation through the ERβ pathway to contribute to lung carcinogenesis in vitro and vivo[J]. Chemosphere, 2020, 263: 127867.
    Sample Type: Cell culture supernatant
  6. Publication: Al‐Hakami A, Alqhatani S Q, Shaik S, et al. Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post‐coculture with activated neutrophils[J]. Journal of Cellular Physiology, 2020.
    Sample Type: Cell culture supernatant
  7. Publication: Rodríguez de Santiago E, Téllez L, Garrido-Lestache Rodríguez-Monte E, et al. Fontan protein‐losing enteropathy is associated with advanced liver disease and a proinflammatory intestinal and systemic state[J]. Liver International, 2020, 40(3): 638-645.
    Sample Type: Plasma
  8. Publication: Brewster L M, Coombs G B, Garcia V P, et al. Effects of circulating extracellular microvesicles from spinal cord-injured adults on endothelial cell function[J]. Clinical Science, 2020, 134(7): 777-789.
    Sample Type: Cell culture supernatant
  9. Publication: Zhang M, Xue Y, Chen H, et al. Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4/NF-κB/STAT3 Activation in Ox-LDL-Treated HUVECs[J]. Oxidative Medicine and Cellular Longevity, 2019, 2019.
    Sample Type: Cell culture supernatant
  10. Publication: Li G Y , Liao Y , Hu J J , et al. Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure[J]. Environmental Pollution, 2019, 252: 1216-1224.
    Sample Type: Cell supernatant
  11. Publication: Li G Y , Liao Y , Hu J J , et al. Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure[J]. Environmental Pollution, 2019, 252: 1216-1224.
    Sample Type: Cell culture supernatant
  12. Publication: Ruan G, Xu J, Wang K, et al. Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis[J]. Osteoarthritis and cartilage, 2018.
    Sample Type: Serum
  13. Publication: Zhang T, Hu Z, Cheng Y, et al. Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin[J]. Journal of Agricultural and Food Chemistry, 2020, 68(13): 4027-4035.
    Sample Type: Serum,Cell culture supernatant
  14. Publication: Yang S, Zhang J, Yan Y, et al. Network Pharmacology-Based Strategy to Investigate the Pharmacologic Mechanisms of Atractylodes macrocephala Koidz. for the Treatment of Chronic Gastritis[J]. Frontiers in Pharmacology, 2020, 10: 1629.
    Sample Type: Cell culture supernatant
  15. Publication: Shen Q, Zheng J, Wang X, et al. LncRNA SNHG5 regulates cell apoptosis and inflammation by miR-132/PTEN axis in COPD[J]. Biomedicine & Pharmacotherapy, 2020, 126: 110016.
    Sample Type: Cell culture supernatant
  16. Publication: Mei J, Zhang Y, Lu S, et al. Long non-coding RNA NNT-AS1 regulates proliferation, apoptosis, inflammation and airway remodeling of chronic obstructive pulmonary disease via targeting miR-582-5p/FBXO11 axis[J]. Biomedicine & Pharmacotherapy, 2020, 129: 110326.
    Sample Type: Cell culture supernatant
  17. Publication: Lobo M G, Schmidt M M, Lopes R D, et al. Treating periodontal disease in patients with myocardial infarction: A randomized clinical trial[J]. European Journal of Internal Medicine, 2020, 71: 76-80.
    Sample Type: Serum
  18. Publication: Lejeune S, Mordacq C, Drumez E, et al. Relationship between immune parameters during a severe exacerbation in allergic asthmatic children and asthma outcomes in the following year[J]. Clinical & Experimental Allergy, 2020, 50(3): 406-411.
    Sample Type: Plasma
  19. Publication: Madkour M I, Obaideen A K, Dalah E Z, et al. Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals[J]. Diabetes research and clinical practice, 2019, 153: 166-175.
    Sample Type: Serum
  20. Publication: Alhazzani A, Rajagopalan P, Albarqi Z, et al. Mesenchymal Stem Cells (MSCs) Coculture Protects [Ca2+] i Orchestrated Oxidant Mediated Damage in Differentiated Neurons In Vitro[J]. Cells, 2018, 7(12): 250.
    Sample Type: Cell culture supernatant
  21. Publication: Park K S, Kim S H, Das A, et al. TLR3-/4-Priming Differentially Promotes Ca2+Signaling And Cytokine Expression And Ca2+-Dependently Augments Cytokine Release In hMSCs[J]. Scientific Reports, 2016.
    Sample Type: Cell culture supernatant
  22. Publication: Lei C T, Su H, Ye C, et al. The Classic Signalling And Trans-signalling Of Interleukin-6 Are Both Injurious In Podocyte Under High Glucose Exposure[J]. Journal of Cellular and Molecular Medicine, 2017.
    Sample Type: Serum
  23. Publication: Zhao Y, Jiang C, He J, et al. Multifunctional Dextran Sulfate-Coated Reconstituted High Density Lipoproteins Target Macrophages And Promote Beneficial Antiatherosclerotic Mechanisms[J]. Bioconjugate Chemistry, 2016.
    Sample Type: Cell culture supernatant
  24. Publication: Yao J, Zhao X, Tan F, et al. Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits[J]. Scientific reports, 2020, 10(1): 1-14.
    Sample Type: Cell culture supernatant
  25. Publication: Wang L, Liu Z, Huang D, et al. IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling Pathway[J]. Mediators of inflammation, 2020, 2020.
    Sample Type: Cell culture supernatant
  26. Publication: Yu H, Liu Y, Xie W, et al. IL-38 Alleviates the Inflammatory Response and the Degeneration of Nucleus Pulposus Cells Via Inhibition of the NF-κB Signaling Pathway In Vitro[J]. International Immunopharmacology, 2020: 106592.
    Sample Type: Cell culture supernatant
  27. Publication: Smok B, Domagalski K, Paw?owska M. Diagnostic and Prognostic Value of IL-6 and sTREM-1 in SIRS and Sepsis in Children[J]. Mediators of Inflammation, 2020, 2020.
    Sample Type: Serum
  28. Publication: Luo N, Gao H M, Wang Y Q, et al. miR-942-5p alleviates septic acute kidney injury by targeting FOXO3[J]. European review for medical and pharmacological sciences, 2020, 24(11): 6237-6244.
    Sample Type: Cell culture supernatant
  29. Publication: Ibrahim Y F, Moussa R A, Bayoumi A M A, et al. Tocilizumab attenuates acute lung and kidney injuries and improves survival in a rat model of sepsis via down-regulation of NF-κB/JNK: a possible role of P-glycoprotein[J]. Inflammopharmacology, 2020, 28(1): 215-230.
    Sample Type: Serum
  30. Publication: Fan Y, Li C, Peng X, et al. Perillaldehyde Ameliorates Aspergillus fumigatus Keratitis by Activating the Nrf2/HO-1 Signaling Pathway and Inhibiting Dectin-1-Mediated Inflammation[J]. Investigative ophthalmology & visual science, 2020, 61(6): 51-51.
    Sample Type: Cell lysate,Cell culture medium
  31. Publication: Almulla A F, Al-Hakeim H K, Abed M S, et al. Chronic fatigue and fibromyalgia symptoms are key components of deficit schizophrenia and are strongly associated with activated immune-inflammatory pathways[J]. Schizophrenia Research, 2020.
    Sample Type: Serum
  32. Publication: Li H, Chen T, Sun H, et al. The first cloned echinoderm tumor necrosis factor receptor from Holothuria leucospilota: Molecular characterization and functional analysis[J]. Fish & Shellfish Immunology, 2019, 93: 542-550.
    Sample Type: Cell culture supernatant
  33. Publication: Li D L, Ye L, Gao L, et al. Plasma concentration of ZCCHC14 contributes to prognostic efficacy in intracerebral hemorrhage patients[J]. European review for medical and pharmacological sciences, 2019, 23(19): 8533-8539.
    Sample Type: Plasma
  34. Publication: Kongdang P, Jaitham R, Thonghoi S, et al. Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways[J]. Phytomedicine, 2018.
    Sample Type: Cell culture supernatant
  35. Publication: Li X, Yu M, Chen L, et al. LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203[J]. Life sciences, 2019, 217: 283-292.
    Sample Type: Cell culture supernatant
  36. Publication: Huang Y, Du K L, Guo P Y, et al. IL-16 regulates macrophage polarization as a target gene of miR-145-3p[J]. Molecular immunology, 2019, 107: 1-9.
    Sample Type: Cell lysate
  37. Publication: Yargic M P, Torgutalp S, Akin S, et al. Acute long-distance trail running increases Serum IL-6, IL-15, and Hsp72 levels[J]. Applied Physiology, Nutrition, and Metabolism, 2018 (ja).
    Sample Type: Serum
  38. Publication: Kong D, Zhang D, Cui Q, et al. Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro[J]. Biomedicine & Pharmacotherapy, 2019, 113: 108706.
    Sample Type: Wound drainage fluid
  39. Publication: Akash M S H, Rehman K, Liaqat A, et al. Biochemical investigation of gender-specific association between insulin resistance and inflammatory biomarkers in types 2 diabetic patients[J]. Biomedicine & Pharmacotherapy, 2018, 106: 285-291.
    Sample Type: Serum
  40. Publication: Kruger M J, Conradie R, Conradie M, et al. ADSC-conditioned media elicit an ex vivo anti-inflammatory macrophage response[J]. Journal of molecular endocrinology, 2018: JME-18-0078.
    Sample Type: Serum, Cell culture medium
  41. Publication: Zhao J, Liang Y, Song F, et al. TSG Attenuates LPC-Induced Endothelial Cells Inflammatory Damage Through Notch Signaling Inhibition[J]. IUBMB Life, 2016, 68(1): 37-50.
    Sample Type: Cell culture supernatant
  42. Publication: Xu H, Sun Q, Lu L, et al. MicroRNA-218 Acts By Repressing TNFR1-Mediated Activation Of NF-κB, Which Is Involved In MUC5AC Hyper-Production And Inflammation In Smoking-Induced Bronchiolitis Of COPD[J]. Toxicology Letters, 2017, 280: 171-180.
    Sample Type: Serum,Cell culture supernatant
  43. Publication: Veena V K, Popavath R N, Kennedy K, et al. In Vitro Antiproliferative, Pro-Apoptotic, Antimetastatic And Anti-Inflammatory Potential Of 2, 4-Diacteylphloroglucinol (DAPG) By Pseudomonas Aeruginosa Strain FP10[J]. Apoptosis, 2015, 20(10): 1281-1295.
    Sample Type: Cell culture supernatant
  44. Publication: Li W, Dong H, Huang Y, et al. Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients[J]. Plos Neglected Tropical Diseases, 2016.
    Sample Type: Serum,Cell culture supernatant
  45. Publication: Tian P, Li B, He C, et al. Antidiabetic (Type 2) Effects Of Lactobacillus G15 And Q14 In Rats Through Regulation Of Intestinal Permeability And Microbiota[J]. Food & Function, 2016.
    Sample Type: Plasma,Serum
  46. Publication: Cheng P, Hua W, Zhang W J, et al. Inhibitory Effect Of miR-125b On Hepatitis C Virus Core Protein-Induced TLR2/MyD88 Signaling In THP-1 Macrophages[J]. World Journal of Gastroenterology, 2016, 22(17): 4354-4361.
    Sample Type: Cell culture supernatant
  47. Publication: Candar T, Asena L, Alkayid H, et al. Galectin-3, IL-1A, IL-6, and EGF Levels in Corneal Epithelium of Patients With Recurrent Corneal Erosion Syndrome[J]. Cornea, 2020, 39(11): 1354-1358.
    Sample Type: Tissue homogenate
  48. Publication: Ma L, Shan W, Ding X, et al. Intermittent hypoxia induces tumor immune escape in murine S180 solid tumors via the upregulation of TGF-β 1 in mice[J]. Sleep and Breathing, 2020: 1-8.
    Sample Type: Tissue homogenate
  49. Publication: Guler E M, Bektay M Y, Akyildiz A G, et al. Investigation of DNA damage, oxidative stress, and inflammation in synthetic cannabinoid users[J]. Human & Experimental Toxicology, 2020: 0960327120930057.
    Sample Type: Plasma
  50. Publication: Sajid M, Biswas K, Singh H, et al. Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes[J]. Autoimmunity, 2020: 1-9.
    Sample Type: Serum
  51. Publication: Wang Q. XIST silencing alleviated inflammation and mesangial cells proliferation in diabetic nephropathy by sponging miR-485[J]. Archives of Physiology and Biochemistry, 2020: 1-7.
    Sample Type: Serum
  52. Publication: Zheng Y, Sun L, Xu M, et al. Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China[J]. Journal of Zhejiang University. Science. B, 2020, 21(5): 378.
    Sample Type: Serum,Plasma
  53. Publication: Yal??n A D, Yal??n A N. A case of Asthma with Beh?et's disease: successful treatment with Omalizumab and its effects on recurrent aphthous lesions[J]. Immunopharmacology and Immunotoxicology, 2020 (just-accepted): 1-12.
    Sample Type: Serum
  54. Publication: Rakhra G, Masih D, Vats A, et al. Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line[J]. Biological Trace Element Research, 2019: 1-10.
    Sample Type: Cell lysate
  55. Publication: Song B, Ye L, Wu S, et al. Long non-coding RNA MEG3 regulates CSE-induced apoptosis and inflammation via regulating miR-218 in 16HBE cells[J]. Biochemical and Biophysical Research Communications, 2020, 521(2): 368-374.
    Sample Type: Cell culture supernatant
  56. Publication: Li H, Chen T, Sun H, et al. Functional characterisation of Holothuria leucospilota Fas-associated death domain in the innate immune-related signalling pathways[J]. Innate Immunity, 2020, 26(2): 138-145.
    Sample Type: Cell culture supernatant
  57. Publication: Jankowski J, Mikulski D, Mikulska M, et al. The effect of different dietary ratios of arginine, methionine, and lysine on the performance, carcass traits, and immune status of turkeys[J]. Poultry Science, 2020, 99(2): 1028-1037.
    Sample Type: Plasma
  58. Publication: Shah M, Ali A, Malik M O, et al. Treatment with metformin and combination of metformin plus pioglitazone on serum levels of IL-6 and IL-8 in polycystic ovary syndrome: a randomized clinical trial[J]. Hormone and Metabolic Research, 2019, 51(11): 714-722.
    Sample Type: Serum
  59. Publication: Martora F, Pinto F, Folliero V, et al. Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles[J]. Microbial pathogenesis, 2019, 136: 103719.
    Sample Type: Cell culture supernatant
  60. Publication: Patra G, Mallik S, Saha B, et al. Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India[J]. Acta tropica, 2019, 190: 73-79.
    Sample Type: Serum
  61. Publication: Jiang L, Zhang S, Hu H, et al. Exosomes derived from human umbilical cord mesenchymal stem cells alleviate acute liver failure by reducing the activity of the NLRP3 inflammasome in macrophages[J]. Biochemical and biophysical research communications, 2019, 508(3): 735-741.
    Sample Type: Serum
  62. Publication: Xiong H, Li W, Li J, et al. Elevated leptin levels in temporomandibular joint osteoarthritis promote proinflammatory cytokine IL-6 expression in synovial fibroblasts[J]. Journal of Oral Pathology & Medicine, 2019, 48(3): 251-259.
    Sample Type: Cell culture supernatant
  63. Publication: Pan Y Y, Zhou F H, Song Z H, et al. Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1-7) upregulation[J]. Biomedicine & Pharmacotherapy, 2018, 97: 1694-1700.
    Sample Type: Cell culture supernatant,Serum
  64. Publication: Liu Q, Wu J B, Song J Q, et al. Particulate Matter 2.5 Regulates Lipid Synthesis And Inflammatory Cytokine Production In Human SZ95 Sebocytes[J]. International Journal of Molecular Medicine, 2017, 40(4): 1029-1036.
    Sample Type: Cell culture supernatant
  65. Publication: Wang N N, Guo J, Liu F D, et al. Depot-Specific Inflammation With Decreased Expression Of ATM2 In White Adipose Tissues Induced By High-Margarine/Lard Intake[J]. Plos One, 2017.
    Sample Type: Cell culture supernatant
  66. Publication: Li Z, Tang Y, Tang N, et al. High Anti-Human Cytomegalovirus Antibody Levels Are Associated With The Progression Of Essential Hypertension And Target Organ Damage In Han Chinese Population[J]. Plos One, 2017.
    Sample Type: Serum
  67. Publication: He W, Ren Y, Wang X, et al. C Reactive Protein And Enzymatically Modified LDL Cooperatively Promote Dendritic Cell-Mediated T Cell Activation[J]. Cardiovascular Pathology, 2017, 29: 1-6.
    Sample Type: Cell culture supernatant
  68. Publication: Hao S Y, Ji J J, Zhao H T,et al. Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation Through Both ROS Scavenging And Inhibition Of Cholesterol Influx In Raw264.7 Cells[J]. Molecules, 2015, 20(12): 21287-21297.
    Sample Type: Cell lysate
  69. Publication: Liao Y, Liu P, Guo F, et al. Oxidative Burst Of Circulating Neutrophils Following Traumatic Brain Injury In Human[J]. Plos One, 2013.
    Sample Type: Plasma
  70. Publication: Huang W K, Xu Q Y, Zhou X, et al. Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1[J]. Archives of Oral Biology, 2020, 118: 104858.
    Sample Type: Cell culture supernatant
  71. Publication: Tiwary S K, Kumar A, Mishra S P, et al. Study of association of varicose veins and inflammation by inflammatory markers[J]. Phlebology, 2020: 0268355520932410.
    Sample Type: Plasma
  72. Publication: Kerget B, Kerget F, Ko?ak A O, et al. Are Serum Interleukin 6 and Surfactant Protein D Levels Associated with the Clinical Course of COVID-19?[J]. Lung, 2020: 1-8.
    Sample Type: Plasma
  73. Publication: Zhong Y, Liu C, Feng J, et al. Curcumin affects ox-LDL-induced IL-6, TNF-α, MCP-1 secretion and cholesterol efflux in THP-1 cells by suppressing the TLR4/NF-κB/miR-3a signaling pathway[J]. Experimental and Therapeutic Medicine, 2020.
    Sample Type: Cell culture supernatant
  74. Publication: Zhang Z, Lv G, Du L. Avicularin Reduces the Expression of Mediators of Inflammation and Oxidative Stress in Bradykinin-Treated MG-63 Human Osteoblastic Osteosarcoma Cells[J]. Medical Science Monitor, 2020, 26.
    Sample Type: Cell culture supernatant
  75. Publication: Liu X, Wang Z, Song W, et al. Systematically transplanted human gingiva-derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis[J]. Experimental and Therapeutic Medicine, 2020, 19(1): 672-682.
    Sample Type: Serum
  76. Publication: Guan Z. Changes in expression of serum chemokine CXCL13 and IL-6 after hip replacement, and the relationship with lower limb vein thrombus[J]. Experimental and Therapeutic Medicine, 2020, 19(3): 2113-2118.
    Sample Type: Serum
  77. Publication: Cao F, Cao P, Liu Y, et al. Effect of laparoscopic sleeve gastrectomy on renal function in obese patients[J]. ANZ Journal of Surgery, 2020, 90(4): 514-520.
    Sample Type: Serum,Urine
  78. Publication: Zhang Y, Li M, Bao L, et al. A case-control study on the relationship between miR-As single nucleotide polymorphisms and sepsis risk[J]. medicine, 2019, 98(33).
    Sample Type: Serum
  79. Publication: Qu N, Zhang S F, Xia B, et al. Sex difference in IL-6 modulation of cognition among Chinese individuals with major depressive disorder[J]. Journal of Clinical Neuroscience, 2019, 70: 14-19.
    Sample Type: Serum
  80. Publication: Zhang C, Yu W, Huang C, et al. Chrysin protects human osteoarthritis chondrocytes by inhibiting inflammatory mediator expression via HMGB1 suppression[J]. Molecular medicine reports, 2019, 19(2): 1222-1229.
    Sample Type: Cell culture medium
  81. Publication: Wei L, Chen Y, Zhang C, et al. Leptin induces IL-6 and IL-8 expression through leptin receptor Ob-Rb in human dental pulp fibroblasts[J]. Acta Odontologica Scandinavica, 2019, 77(3): 205-212.
    Sample Type: Cell culture supernatant
  82. Publication: Liu R, Xu F, Bi S, et al. Mitochondrial DNA-Induced Inflammatory Responses and Lung Injury in Thermal Injury Murine Model: Protective Effect of Cyclosporine-A[J]. Journal of Burn Care & Research, 2019.
    Sample Type: Serum
  83. Publication: Han S L, Zhang Y J, Zhou M, et al. Association of PAR-2 gene polymorphisms with the inflammatory response and susceptibility to knee osteoarthritis in the Chinese Han population[J]. Genetic testing and molecular biomarkers, 2019, 23(2): 84-90.
    Sample Type: Synovia
  84. Publication: Cao D, Pi J, Shan Y, et al. Anti-inflammatory Effect of Resolvin D1 on LPS-treated MG-63 Cells[J]. Experimental and therapeutic medicine, 2018, 16(5): 4283-4288.
    Sample Type: Serum
  85. Publication: Wang Y, Qiu R, Kong G, et al. Effects of propofol combined with remifentanil anesthesia on the NO, endothelin and inflammatory cytokines in the plasma of patients with liver cirrhosis during the perioperative period[J]. Experimental and Therapeutic Medicine.
    Sample Type: Plasma
  86. Publication: Jiang Z, Wang W H, Guo C C. Tetrahydroxy Stilbene Glucoside Ameliorates H2O2-Induced Human Brain Microvascular Endothelial Cell Dysfunction In Vitro By Inhibiting Oxidative Stress And Inflammatory Responses[J]. Molecular Medicine Reports, 2017, 16(4): 5219-5224.
    Sample Type: Cell culture supernatant
  87. Publication: Lu A Q, Wang Z R, Wang S C. Role Of miR-589-3p In Human Lumbar Disc Degeneration And Its Potential Mechanism[J]. Experimental and Therapeutic Medicine, 2018, 15: 1616-1621.
    Sample Type: Cell culture supernatant
  88. Publication: Cao Z, Chen L. Inhibition Of miR27a Suppresses The Inflammatory Response Via The p38/MAPK Pathway In Intervertebral Disc Cells[J]. Experimental and Therapeutic Medicine, 2017, 14(5): 4572-4578.
    Sample Type: Cell culture supernatant
  89. Publication: Tang J N, Shen L, Liu C L, et al. Plasma Levels Of C1q/TNF-Related Protein 1 And Interleukin 6 In Patients With Acute Coronary Syndrome Or Stable Angina Pectoris[J]. American Journal of The Medical Sciences, 2015, 349(2): 130-136.
    Sample Type: Plasma
  90. Publication: Sahin O N, Ozpinar A, Serdar M. Maternal omega-3 polyunsaturated fatty acids supplementation in pregnancy decreases MMP-1 levels in breastmilk: a cross-sectional study[J]. The Journal of Maternal-Fetal & Neonatal Medicine, 2020: 1-9.
    Sample Type: Milk
  91. Publication: Jasim A E, Majeed S A, Hadi N R, et al. Atorvastatin Reload Down Regulates TLR-2 Expression and Reduces the Acute Inflammatory Response in Patients Undergoing Percutaneous Coronary Intervention[J]. Systematic Reviews in Pharmacy, 2020, 11(2): 347-355.
    Sample Type: Serum
  92. Publication: Iduh P D, Adias T C, Eze E M. Evaluation of Cytokines Response in Plasmodium falciparum Infection among Adolescents in Port Harcourt, Nigeria[J]. JMSCR,2018.
    Sample Type: Plasma
  93. Publication: Al-Harbi S J, Rashid K H, Hadi M A. Association of Interleukin-6 Level with Some Clinical Parameters in Iraqi Juvenile Idiopathic Arthritis Patients[J]. Indian Journal of Public Health Research & Development, 2019, 10(10): 2119-2124.
    Sample Type: Serum
  94. Publication: Kadhem S A, Rabee A M. STUDY OF SOME IMMUNOLOGICAL PARAMETERS FOR EXPOSURE TO PARTICULATE MATTER IN THE POPULATION OF BAGHDAD[J].Plant Archives, 2020.
    Sample Type: Serum
  95. Publication: Domngang C, Noutchie A L, Chuisseu P D, et al. Level of VEGF-A and interleukin 6 in lacrimal fluid of patients with diabetic retinopathy[J]. International Journal of Biological and Chemical Sciences, 2020, 14(3): 664-673.
    Sample Type: Tears
  96. Publication: Latjindung N, Ganis Siregar M F, Hartono H, et al. Correlation of Interleukin-6 with Serum Estradiol Mean Levels in Menopause Women at Rsup H Adam Malik Medan[J]. Indian Journal of Public Health Research & Development, 2020, 11(1).
    Sample Type: Serum
  97. Publication: Lai N, Yao Y, Qin F, et al. Increased interleukin-6 levels in neuron-derived plasma small extracellular vesicles in patients with aneurysmal subarachnoid hemorrhage[J]. Preprint, 2020.
    Sample Type: Plasma
  98. Publication: Zeng J, Yang L, Zheng Y. Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma[J]. Preprint, 2020.
    Sample Type: Cell culture supernatant
  99. Publication: Noche C D, Kouosseu F N, Bilong Y, et al. Relationship between Angiogenic and Inflammatory Biomarkers and Diabetic Retinopathy[J]. Open Journal of Ophthalmology, 2020, 10(01): 44.
    Sample Type: Serum
  100. Publication: Sheng C, Wang Z, Yao C, et al. CALML6 Controls TAK1 Ubiquitination and Confers Protection against Acute Inflammation[J]. The Journal of Immunology, 2020, 204(11): 3008-3018.
    Sample Type: Cell culture supernatant
  101. Publication: AbdullahAbbas A, Saadoon I H. Relation of Epstein Barr virus with Interleukin-6 Level among Women with Breast Cancer in Ramadi City[J]. Medico Legal Update, 2020, 20(1): 1393-1398.
    Sample Type: Plasma
  102. Publication: Pramudji H, Demes C M, Dewi K, et al. Association of-174 G> C interleukin-6 gene polymorphism with interleukin-6 and c-reactive protein levels and obesity: A case-control study among people/residents of Western Indonesia[J]. The Medical journal of Malaysia, 2019, 74(5): 400-404.
    Sample Type: Plasma
  103. Publication: Huang Y, Zhou F, Xiao Y, et al. TLR7 mediates increased vulnerability to ischemic acute kidney injury in diabetes[J]. Revista da Associa??o Médica Brasileira, 2019, 65(8): 1067-1073.
    Sample Type: Cell culture supernatant
  104. Publication: Al-mudhaffar M H, Zaidan T F, Gorial F I. Evaluation of IL-6 and TGF-β1level in serum and saliva of patients with systemic sclerosis[J].IOSR Journal of Dental and Medical Sciences, 2016, 15(09):88-92.
    Sample Type: Serum,Saliva
  105. Publication: Sun H B, Li Y, Liu X B, et al. Impact of an Early Oral Feeding Protocol on Inflammatory Cytokine Changes After Esophagectomy[J]. The Annals of thoracic surgery, 2019, 107(3): 912-920.
    Sample Type: Serum
  106. Publication: Faizal1ab I A, Nugraha A D W W J. The Effect of Macrophage Dose To Secretion Interleukin 6 (IL-6) On Model of Tuberculosis Granuloma In Vitro[J].
    Sample Type: Cell culture supernatant
  107. Publication: Pratiwi W N, Purwanto B. Diabetes dance of persadia 1 effect on blood IL-6 level[C]//Journal of Physics: Conference Series. IOP Publishing, 2019, 1146(1): 012014.
    Sample Type: Serum
  108. Publication: Al-Jebouri M M, Al-Mahmood B Y R. Estimation of Cytokines Involved in Acute-Phase Wound Infection with Reference to Residence Time of Patients in Hospitals[J]. 2019.
    Sample Type: Serum
  109. Publication: Shen J, Yao R, Jing M, et al. Sinomenine Regulates Inflammatory Response and Oxidative Stress via Nuclear Factor kappa B (NF-kB) and NF-E2-Related Factor 2 (Nrf2) Signaling Pathways in Ankle Fractures in Children[J]. Medical science monitor: international medical journal of experimental and clinical research, 2018, 24: 6649.
    Sample Type: Serum
  110. Publication: AZIZ T A. EFFECTS OF GINKGO BILOBA EXTRACT (EGb761) AS ADD-ON WITH METFORMIN IN THE TREATMENT OF PATIENTS WITH METABOLIC SYNDROME AND TYPE 2 DIABETES MELLITUS IN SULAIMANI[D]. University of Sulaimani, 2018.
    Sample Type: Serum
  111. Publication: Zhou P, Liu H, Wu Y, et al. Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response[J]. Medical Science Monitor: International Medical Journal of Experimental And Clinical Research, 2018, 24: 4379.
    Sample Type: Serum
  112. Publication: Wang F, Li X, Huang L, et al. High-Mobility Group Protein Box 1 is Upregulated in Children with Henoch-Schonlein Purpura[J]. Pediatric Allergy, Immunology, and Pulmonology, 2018, 31(2): 66-72.
    Sample Type: Serum
  113. Publication: Li L, Zhang J, Zhao M, et al. Applied Value Of Monitoring Serum Hepcidin In Differential Diagnosis Of Infection Versus Tumor Fevers[J]. Journal of Huazhong University of Science and Technology-Medical Sciences, 2017, 37(2): 253-256.
    Sample Type: Plasma
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