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Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit (E-EL-H0109)

  • +1
AllSizePriceQty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity4.69 pg/mL
Detection Range7.81-500 pg/mL
Sample Volume100 μL
Total Assay Time3 h 30 min
ReacitivityHuman
SpecificityThis kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed
Recovery80%-120%
Sample TypeSerum, plasma and other biological fluids
Detection MethodColorimetric method, ELISA, Sandwich
Assay TypeSandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage2-8℃
Expiration Date12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNF-α. You can calculate the concentration of Human TNF-α in the samples by comparing the OD of the samples to the standard curve.
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        • Q1:Why is it necessary to add a protease inhibitor in tissue sample preparation during an Elisa experiment? Will it affect the detection significantly if there is no protease inhibitor?

          Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.

        • Q2:My sample volume is small. Can I reduce some reagents proportionally?

          No, the detection system of our kits requires strict adherence to the specified sample volume to ensure accurate detection. If the sample volume is insufficient, consider diluting appropriately, but first conduct a pre-experiment to confirm the suitable dilution factor.

        • Q3:I need to measure corticosterone and testosterone in hair samples. Are there any suggested sample extraction methods?

          Hair sample preparation method: Wash hair samples with methanol by adding 5 mL of HPLC-grade methanol to each sample, rotating for 3 minutes, then decanting excess methanol and rinsing hair twice. After washing, place the hair samples on aluminum foil, dry for 3 days in a protective cap. Weigh the dried hair samples and transfer them to 2ml polypropylene tubes containing stainless steel grinding beads. Place the tubes containing hair and beads in a bead beater, grind each sample for 2 minutes to produce powder. After grinding, add 1.5 mL of methanol to the tubes containing hair powder. Rotate samples slowly for 24 hours to extract steroids. Centrifuge at 10000 g for 4 minutes, transfer 0.6 mL of methanol supernatant containing steroids to new 1.5 mL microcentrifuge tubes. Dry the samples in a protective hood for 2-3 days to evaporate the methanol. Dilute the dried extract with 0.4 mL dilution buffer from the kit for detection.

        • Q4:Do your ELISA kits have the enzyme that catalyzes the substrate reaction is not HRP? My sample is red blood cell lysate, and HRP will interfere with hemoglobin. Or is there a way to remove Hb?

          The detection antibodies in our ELISA kits are all HRP-based. Currently, there are no kits available for testing red blood cell lysate. For small amounts of hemoglobin, consider increasing the number of washing steps by 1-2 during the first plate washing step to reduce interference. However, besides hemoglobin, substances such as endogenous HRP enzymes released during the process of red blood cell lysis can also catalyze the substrate, so we still do not recommend testing with such samples.

        • Q5:Do you have ELISA kits for plant or microbial samples?

          Currently, our ELISA kits are only suitable for animal samples, and we do not have kits for testing plant/microbial samples.

        • Q6:Do you have a suggested preparation method for vaginal discharge samples?

          After the collection with the swab, elute with 500μl PBS, centrifuge at 5000×g for 10 minutes (if the sample remains turbid, try centrifuging at 10000×g), then collect the supernatant for testing.

        • Q7:Can ELISA kits be used on automated analyzers?

          Our ELISA kits have not been validated for compatibility with automated analyzers. The provided instructions are for manual operation. Customers may validate them on their own if needed.

        • Q8:Can a shaker or a water bath be used during the incubation stage in ELISA experiments?

          To ensure temperature fluctuations during incubation stay within 1°C, it's recommended to use an incubator. If an incubator is not available, a water bath may be used, but precautions should be taken to avoid contaminating the plate with water. Using a shaker, especially one with vibration, for incubation is not recommended.