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Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

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Qty:
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Price:   $495
Cat Number:  E-EL-H0109
Lead Time:   7~10 days

Reactivity:  Human

Detection Range:  7.81~500 pg/mL

Sensitivity:  4.69 pg/mL

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Intended use

This ELISA kit applies to the in vitro quantitative determination of Human TNF-α concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNF-α. You can calculate the concentration of Human TNF-α in the samples by comparing the OD of the samples to the standard curve.

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Reactivity Human
Detection Method Colormetric
Detection Range 7.81—500 pg/mL
Sensitivity 4.69 pg/mL
Sample Volume Required Per Well 100μL
Sample Type serum, plasma and other biological fluids

Specificity

This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration
(pg/mL)
O.D Average Corrected
500 2.427
2.445
2.436 2.376
250 1.562
1.584
1.573 1.513
125 0.993
0.979
0.986 0.926
62.5 0.477
0.515
0.496 0.436
31.25 0.245
0.237
0.241 0.181
15.63 0.166
0.16
0.163 0.103
7.81 0.105
0.121
0.113 0.053
0 0.059
0.061
0.06 --

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TNF-α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TNF-α were tested on 3 different plates, 20 replicates in each plate.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 24.10 79.50 183.00 22.70 86.50 177.40
Standard deviation 1.50 3.80 9.70 1.20 4.00 7.60
C V (%) 6.22 4.78 5.30 5.29 4.62 4.28

Recovery

The recovery of Human TNF-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum (n=5) 86-100 92
EDTA plasma (n=5) 94-106 99
Cell culture media (n=5) 93-107 99

Linearity

Samples were spiked with high concentrations of Human TNF-α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 92-105 84-97 88-99
Average (%) 99 91 93
1:4 Range (%) 99-116 87-99 91-104
Average (%) 107 93 98
1:8 Range (%) 97-113 81-93 92-109
Average (%) 104 88 99
1:16 Range (%) 96-112 85-98 95-107
Average (%) 103 90 100

Kit components & Storage

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4℃(shading light)
Stop Solution 1 vial, 10 mL 4℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

  • Microplate reader with 450 nm wavelength filter
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37℃
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

Assay Procedure

1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

2.Remove the liquid.

3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

6.Add 50 μL Stop Solution.

7.Read at 450 nm immediately. Calculation of results.

Citations

  1. Publication: Zhao Q, Liu J, Deng H, et al. Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output[J]. Cell, 2020.
    Sample Type: Cell culture medium
  2. Publication: Carpino G, Del Ben M, Pastori D, et al. Increased Liver Localization of Lipopolysaccharides in Human and Experimental NAFLD[J]. Hepatology, 2020.
    Sample Type: Serum
  3. Publication: Chen W H, Luo G F, Lei Q, et al. Overcoming The Heat Endurance Of Tumor Cells By Interfering The Anaerobic Glycolysis Metabolism For Improved Photothermal Therapy[J]. Acs Nano, 2017, 11(2): 1419-1431.
    Sample Type: Cell culture supernatant
  4. Publication: Singh G, Nayal A, Malhotra S, et al. Dual functionalized chitosan based composite hydrogel for haemostatic efficacy and adhesive property[J]. Carbohydrate Polymers, 2020, 247: 116757.
    Sample Type: Cell culture supernatant
  5. Publication: Montico B, Lapenta C, Ravo M, et al. Exploiting A New Strategy To Induce Immunogenic Cell Death To Improve Dendritic Cell-Based Vaccines For Lymphoma Immunotherapy[J]. OncoImmunology, 2017.
    Sample Type: Cell culture supernatant
  6. Publication: Ilmiawati C, Fitri F, Rofinda Z D, et al. Green coffee extract modifies body weight, serum lipids and TNF-α in high-fat diet-induced obese rats[J]. BMC Research Notes, 2020, 13(1): 1-5.
    Sample Type: Serum
  7. Publication: Huang Z, Du G, Huang X, et al. The enhancer RNA lnc-SLC4A1-1 epigenetically regulates unexplained recurrent pregnancy loss (URPL) by activating CXCL8 and NF-kB pathway[J]. EBioMedicine, 2018, 38: 162-170.
    Sample Type: Cell culture supernatant
  8. Publication: Al‐Hakami A, Alqhatani S Q, Shaik S, et al. Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post‐coculture with activated neutrophils[J]. Journal of Cellular Physiology, 2020.
    Sample Type: Cell culture supernatant
  9. Publication: Rodríguez de Santiago E, Téllez L, Garrido-Lestache Rodríguez-Monte E, et al. Fontan protein‐losing enteropathy is associated with advanced liver disease and a proinflammatory intestinal and systemic state[J]. Liver International, 2020, 40(3): 638-645.
    Sample Type: Plasma
  10. Publication: Brewster L M, Coombs G B, Garcia V P, et al. Effects of circulating extracellular microvesicles from spinal cord-injured adults on endothelial cell function[J]. Clinical Science, 2020, 134(7): 777-789.
    Sample Type: Cell culture supernatant
  11. Publication: Ma L, She C, Shi Q, et al. TNFα inhibitor C87 sensitizes EGFRvIII transfected glioblastoma cells to gefitinib by a concurrent blockade of TNFα signaling[J]. Cancer Biology & Medicine, 2019, 16(3): 606.
    Sample Type: Cell sample
  12. Publication: Ruan G, Xu J, Wang K, et al. Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis[J]. Osteoarthritis and cartilage, 2018.
    Sample Type: Serum
  13. Publication: Liu N, Sun Q, Xu H, et al. Hyperuricemia induces lipid disturbances mediated by LPCAT3 upregulation in the liver[J]. The FASEB Journal, 2020.
    Sample Type: Cell culture supernatant
  14. Publication: Yang Y, Liu S, Liu J, et al. Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kβsignaling[J]. Journal of Reproductive Immunology, 2020: 103192.
    Sample Type: Cell culture supernatant
  15. Publication: Shen Q, Zheng J, Wang X, et al. LncRNA SNHG5 regulates cell apoptosis and inflammation by miR-132/PTEN axis in COPD[J]. Biomedicine & Pharmacotherapy, 2020, 126: 110016.
    Sample Type: Cell culture supernatant
  16. Publication: Mei J, Zhang Y, Lu S, et al. Long non-coding RNA NNT-AS1 regulates proliferation, apoptosis, inflammation and airway remodeling of chronic obstructive pulmonary disease via targeting miR-582-5p/FBXO11 axis[J]. Biomedicine & Pharmacotherapy, 2020, 129: 110326.
    Sample Type: Cell culture supernatant
  17. Publication: Madkour M I, Obaideen A K, Dalah E Z, et al. Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals[J]. Diabetes research and clinical practice, 2019, 153: 166-175.
    Sample Type: Serum
  18. Publication: Hu Y, Hong X Y, Yang X F, et al. Inflammation-dependent ISG15 upregulation mediates MIA-induced dendrite damages and depression by disrupting NEDD4/Rap2A signaling[J]. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 2019.
    Sample Type: Serum
  19. Publication: Yan Z, Wang W, Wu Y, et al. Zinc Oxide Nanoparticle-Induced Atherosclerotic Alterations In Vitro And In Vivo[J]. International Journal of Nanomedicine, 2017, 12: 4433-4442.
    Sample Type: Cell culture supernatant,Serum
  20. Publication: Jiang Y, Kou J, Han X, et al. Ros-Dependent Activation Of Autophagy Through The PI3K/Akt/mTOR Pathway Is Induced By Hydroxysafflor Yellow A-Sonodynamic Therapy In THP-1 Macrophages[J]. Oxidative Medicine and Cellular Longevity, 2017.
    Sample Type: Cell culture supernatant
  21. Publication: Tang Y, Zhang Y C, Chen Y, et al. The Role Of miR-19b In The Inhibition Of Endothelial Cell Apoptosis And Its Relationship With Coronary Artery Disease[J]. Scientific Reports, 2015.
    Sample Type: Serum
  22. Publication: Zhao Y, Jiang C, He J, et al. Multifunctional Dextran Sulfate-Coated Reconstituted High Density Lipoproteins Target Macrophages And Promote Beneficial Antiatherosclerotic Mechanisms[J]. Bioconjugate Chemistry, 2016.
    Sample Type: Cell culture supernatant
  23. Publication: Zhang X P, Shang W L, Yuan J Z, et al. Positive Feedback Cycle Of TNF-α Promotes Staphylococcal Enterotoxin B-Induced THP-1 Cell Apoptosis[J]. Frontier in Cellular and Infection Microbiology, 2016.
    Sample Type: Cell culture supernatant
  24. Publication: Yao J, Zhao X, Tan F, et al. Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits[J]. Scientific reports, 2020, 10(1): 1-14.
    Sample Type: Cell culture supernatant
  25. Publication: Li H, Wu X, Chen T, et al. Molecular characterization, inducible expression and functional analysis of an IKKβ from the tropical sea cucumber Holothuria leucospilota[J]. Fish & Shellfish Immunology, 2020, 104: 622-632.
    Sample Type: Cell culture supernatant
  26. Publication: Abdel-Aziz A M, El-Tahawy N F G, Mohammed M M, et al. Amelioration of testosterone-induced benign prostatic hyperplasia using febuxostat in rats: The role of VEGF/TGFβ and iNOS/COX-2[J]. European Journal of Pharmacology, 2020: 173631.
    Sample Type: Serum,Cell sample
  27. Publication: Liu C, He L, Wang J, et al. Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways[J]. Journal of Ethnopharmacology, 2020: 113039.
    Sample Type: Serum,Cell culture supernatant
  28. Publication: Luo N, Gao H M, Wang Y Q, et al. miR-942-5p alleviates septic acute kidney injury by targeting FOXO3[J]. European review for medical and pharmacological sciences, 2020, 24(11): 6237-6244.
    Sample Type: Cell culture supernatant
  29. Publication: Jiang X, Liu W, Wu Y, et al. Notopterygium incisum extract (NRE) rescues cognitive deficits in APP/PS1 Alzhneimer's disease mice by attenuating amyloid-beta, tau, and neuroinflammation pathology[J]. Journal of Ethnopharmacology, 2020, 249: 112433.
    Sample Type: Cell culture supernatant
  30. Publication: Ibrahim Y F, Moussa R A, Bayoumi A M A, et al. Tocilizumab attenuates acute lung and kidney injuries and improves survival in a rat model of sepsis via down-regulation of NF-κB/JNK: a possible role of P-glycoprotein[J]. Inflammopharmacology, 2020, 28(1): 215-230.
    Sample Type: Serum
  31. Publication: Fan Y, Li C, Peng X, et al. Perillaldehyde Ameliorates Aspergillus fumigatus Keratitis by Activating the Nrf2/HO-1 Signaling Pathway and Inhibiting Dectin-1-Mediated Inflammation[J]. Investigative ophthalmology & visual science, 2020, 61(6): 51-51.
    Sample Type: Cell lysate,Cell culture medium
  32. Publication: Almulla A F, Al-Hakeim H K, Abed M S, et al. Chronic fatigue and fibromyalgia symptoms are key components of deficit schizophrenia and are strongly associated with activated immune-inflammatory pathways[J]. Schizophrenia Research, 2020.
    Sample Type: Serum
  33. Publication: Li D L, Ye L, Gao L, et al. Plasma concentration of ZCCHC14 contributes to prognostic efficacy in intracerebral hemorrhage patients[J]. European review for medical and pharmacological sciences, 2019, 23(19): 8533-8539.
    Sample Type: Plasma
  34. Publication: Gao, J., Kong, R., Zhou, X. et al. miR-A-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis. Apoptosis 23, 607-615 (2018).
    Sample Type: Serum
  35. Publication: Zheng Z, Zeng Y Z, Ren K, et al. S1P promotes inflammation-induced tube formation by HLECs via the S1PR1/NF-κB pathway[J]. International immunopharmacology, 2019, 66: 224-235.
    Sample Type: Cell culture supernatant
  36. Publication: Li X, Yu M, Chen L, et al. LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203[J]. Life sciences, 2019, 217: 283-292.
    Sample Type: Cell culture supernatant
  37. Publication: Huang Y, Du K L, Guo P Y, et al. IL-16 regulates macrophage polarization as a target gene of miR-145-3p[J]. Molecular immunology, 2019, 107: 1-9.
    Sample Type: Cell lysate
  38. Publication: Kong D, Zhang D, Cui Q, et al. Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro[J]. Biomedicine & Pharmacotherapy, 2019, 113: 108706.
    Sample Type: Wound drainage fluid
  39. Publication: Akash M S H, Rehman K, Liaqat A, et al. Biochemical investigation of gender-specific association between insulin resistance and inflammatory biomarkers in types 2 diabetic patients[J]. Biomedicine & Pharmacotherapy, 2018, 106: 285-291.
    Sample Type: Serum
  40. Publication: Yu H, Dong R, Lu Y Y, et al. Short-Term Postoperative Cognitive Dysfunction And Inflammatory Response In Patients Undergoing Cytoreductive Surgery And Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study[J]. Mediators Of Inflammation, 2017.
    Sample Type: Serum
  41. Publication: Belvedere R, Bizzarro V, Parente L, et al. The Pharmaceutical Device Prisma Skin Promotes In Vitro Angiogenesis Through Endothelial To Mesenchymal Transition During Skin Wound Healing[J]. International Journal of Molecular Sciences, 2017.
    Sample Type: Cell culture supernatant
  42. Publication: Veena V K, Popavath R N, Kennedy K, et al. In Vitro Antiproliferative, Pro-Apoptotic, Antimetastatic And Anti-Inflammatory Potential Of 2, 4-Diacteylphloroglucinol (DAPG) By Pseudomonas Aeruginosa Strain FP10[J]. Apoptosis, 2015, 20(10): 1281-1295.
    Sample Type: Cell culture supernatant
  43. Publication: Cheng P, Hua W, Zhang W J, et al. Inhibitory Effect Of miR-125b On Hepatitis C Virus Core Protein-Induced TLR2/MyD88 Signaling In THP-1 Macrophages[J]. World Journal of Gastroenterology, 2016, 22(17): 4354-4361.
    Sample Type: Cell culture supernatant
  44. Publication: Ma L, Shan W, Ding X, et al. Intermittent hypoxia induces tumor immune escape in murine S180 solid tumors via the upregulation of TGF-β 1 in mice[J]. Sleep and Breathing, 2020: 1-8.
    Sample Type: Tissue homogenate
  45. Publication: Iyigun I, Alikasifoglu A, Gonc N, et al. Obstructive sleep apnea in children with hypothalamic obesity: Evaluation of possible related factors[J]. Pediatric Pulmonology, 2020.
    Sample Type: Plasma
  46. Publication: Guler E M, Bektay M Y, Akyildiz A G, et al. Investigation of DNA damage, oxidative stress, and inflammation in synthetic cannabinoid users[J]. Human & Experimental Toxicology, 2020: 0960327120930057.
    Sample Type: Plasma
  47. Publication: Sajid M, Biswas K, Singh H, et al. Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes[J]. Autoimmunity, 2020: 1-9.
    Sample Type: Serum
  48. Publication: Wang Q. XIST silencing alleviated inflammation and mesangial cells proliferation in diabetic nephropathy by sponging miR-485[J]. Archives of Physiology and Biochemistry, 2020: 1-7.
    Sample Type: Serum
  49. Publication: Zheng Y, Sun L, Xu M, et al. Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China[J]. Journal of Zhejiang University. Science. B, 2020, 21(5): 378.
    Sample Type: Serum,Plasma
  50. Publication: Rakhra G, Masih D, Vats A, et al. Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line[J]. Biological Trace Element Research, 2019: 1-10.
    Sample Type: Cell lysate
  51. Publication: Song B, Ye L, Wu S, et al. Long non-coding RNA MEG3 regulates CSE-induced apoptosis and inflammation via regulating miR-218 in 16HBE cells[J]. Biochemical and Biophysical Research Communications, 2020, 521(2): 368-374.
    Sample Type: Cell culture supernatant
  52. Publication: Olusola B A, Kabelitz D, Olaleye D O, et al. Early HIV infection is associated with reduced proportions of gamma delta T subsets as well as high creatinine and urea levels[J]. Scandinavian Journal of Immunology, 2020, 91(5): e12868.
    Sample Type: Serum
  53. Publication: Maharshi V, Gupta P, Kumar V L, et al. Effect of Helicobacter pylori-eradication therapy on hepatic steatosis in patients with non-alcoholic fatty liver disease: a randomized–controlled pilot study[J]. Gastroenterology Report, 2020, 8(2): 104-110.
    Sample Type: Serum
  54. Publication: Jankowski J, Mikulski D, Mikulska M, et al. The effect of different dietary ratios of arginine, methionine, and lysine on the performance, carcass traits, and immune status of turkeys[J]. Poultry Science, 2020, 99(2): 1028-1037.
    Sample Type: Plasma
  55. Publication: Al-Hakeim H K, Najm A H, Al-Dujaili A H, et al. Major Depression in Children with Transfusion-Dependent Thalassemia Is Strongly Associated with the Combined Effects of Blood Transfusion Rate, Iron Overload, and Increased pro-inflammatory Cytokines[J]. Neurotoxicity research, 2020, 38(1): 228-241.
    Sample Type: Serum
  56. Publication: Martora F, Pinto F, Folliero V, et al. Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles[J]. Microbial pathogenesis, 2019, 136: 103719.
    Sample Type: Cell culture supernatant
  57. Publication: Gonciarz W, Matusiak A, Rudnicka K, et al. Autoantibodies to a specific peptide epitope of human Hsp60 (ATVLA) with homology to Helicobacter pylori HspB in H. pylori-infected patients. APMIS. 2019;127(3):139-149.
    Sample Type: Cell culture supernatant
  58. Publication: López Z, Femenia A, Nú?ez-Jinez G, et al. In Vitro Immunomodulatory Effect of Food Supplement from Aloe vera. Evid Based Complement Alternat Med. 2019;2019:5961742. Published 2019 Mar 3.
    Sample Type: Cell culture supernatant
  59. Publication: Pan Y Y, Zhou F H, Song Z H, et al. Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1-7) upregulation[J]. Biomedicine & Pharmacotherapy, 2018, 97: 1694-1700.
    Sample Type: Cell culture supernatant,Serum
  60. Publication: Li Z, Tang Y, Tang N, et al. High Anti-Human Cytomegalovirus Antibody Levels Are Associated With The Progression Of Essential Hypertension And Target Organ Damage In Han Chinese Population[J]. Plos One, 2017.
    Sample Type: Serum
  61. Publication: He W, Ren Y, Wang X, et al. C Reactive Protein And Enzymatically Modified LDL Cooperatively Promote Dendritic Cell-Mediated T Cell Activation[J]. Cardiovascular Pathology, 2017, 29: 1-6.
    Sample Type: Cell culture supernatant
  62. Publication: Hao S Y, Ji J J, Zhao H T,et al. Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation Through Both ROS Scavenging And Inhibition Of Cholesterol Influx In Raw264.7 Cells[J]. Molecules, 2015, 20(12): 21287-21297.
    Sample Type: Cell lysate
  63. Publication: Liu Q, Xu C, Ji G, et al. Sublethal Effects Of Zinc Oxide Nanoparticles On Male Reproductive Cells[J]. Toxicology in Vitro, 2016, 35: 131-138.
    Sample Type: Cell culture supernatant
  64. Publication: Cheng P, Hua W, Zhang W, et al. Decreased Monocyte Subsets And TLR4-Mediated Functions In Patients With Acute Severe Fever With Thrombocytopenia Syndrome (SFTS)[J]. International Journal of Infectious Diseases, 2015, 43: 37-42.
    Sample Type: Cell culture supernatant
  65. Publication: Liao Y, Liu P, Guo F, et al. Oxidative Burst Of Circulating Neutrophils Following Traumatic Brain Injury In Human[J]. Plos One, 2013.
    Sample Type: Plasma
  66. Publication: Du X, Chen Q, Song J, et al. Rhubarb-Aconite Decoction (RAD) Drug-Containing Serum Alleviated Endotoxin-Induced Oxidative Stress Injury and Inflammatory Response in Caco-2 Cells In Vitro[J]. Evidence-Based Complementary and Alternative Medicine, 2020, 2020.
    Sample Type: Cell culture supernatant
  67. Publication: Huang W K, Xu Q Y, Zhou X, et al. Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1[J]. Archives of Oral Biology, 2020, 118: 104858.
    Sample Type: Cell culture supernatant
  68. Publication: Liu W, Zhou J, Niu F, et al. Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF-α-mediated osteoclast autophagy[J]. Experimental and therapeutic medicine, 2020, 20(3): 1889-1898.
    Sample Type: Cell culture supernatant
  69. Publication: Sun J, Cai X, Shen J, et al. Correlation Between Single Nucleotide Polymorphisms at the 3′-UTR of the NFKB1 Gene and Acute Kidney Injury in Sepsis[J]. Genetic Testing and Molecular Biomarkers, 2020, 24(5): 274-284.
    Sample Type: Cell sample
  70. Publication: Zhang Z, Lv G, Du L. Avicularin Reduces the Expression of Mediators of Inflammation and Oxidative Stress in Bradykinin-Treated MG-63 Human Osteoblastic Osteosarcoma Cells[J]. Medical Science Monitor, 2020, 26.
    Sample Type: Cell culture supernatant
  71. Publication: Liu X, Wang Z, Song W, et al. Systematically transplanted human gingiva-derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis[J]. Experimental and Therapeutic Medicine, 2020, 19(1): 672-682.
    Sample Type: Serum
  72. Publication: Cao F, Cao P, Liu Y, et al. Effect of laparoscopic sleeve gastrectomy on renal function in obese patients[J]. ANZ Journal of Surgery, 2020, 90(4): 514-520.
    Sample Type: Serum,Urine
  73. Publication: Burcu Ayd?n E. A label-free and sensitive impedimetric immunosensor for TNF-α biomarker detection based on epoxysilane-modified disposable ITO-PET electrode[J]. International Journal of Environmental Analytical Chemistry, 2020, 100(4): 363-377.
    Sample Type: Immunosensor Construction
  74. Publication: Zhang Y, Li M, Bao L, et al. A case-control study on the relationship between miR-As single nucleotide polymorphisms and sepsis risk[J]. medicine, 2019, 98(33).
    Sample Type: Serum
  75. Publication: Song W, Tan H, Wang S, et al. Association of High Mobility Group Box Protein B1 Gene Polymorphisms with Pneumonia Susceptibility and Severity[J]. Genetic testing and molecular biomarkers, 2018, 23(1): 3-11.
    Sample Type: Plasma
  76. Publication: Liu R, Xu F, Bi S, et al. Mitochondrial DNA-Induced Inflammatory Responses and Lung Injury in Thermal Injury Murine Model: Protective Effect of Cyclosporine-A[J]. Journal of Burn Care & Research, 2019.
    Sample Type: Serum
  77. Publication: Cao D, Pi J, Shan Y, et al. Anti-inflammatory Effect of Resolvin D1 on LPS-treated MG-63 Cells[J]. Experimental and therapeutic medicine, 2018, 16(5): 4283-4288.
    Sample Type: Serum
  78. Publication: Wang Y, Qiu R, Kong G, et al. Effects of propofol combined with remifentanil anesthesia on the NO, endothelin and inflammatory cytokines in the plasma of patients with liver cirrhosis during the perioperative period[J]. Experimental and Therapeutic Medicine.
    Sample Type: Plasma
  79. Publication: Jiang Z, Wang W H, Guo C C. Tetrahydroxy Stilbene Glucoside Ameliorates H2O2-Induced Human Brain Microvascular Endothelial Cell Dysfunction In Vitro By Inhibiting Oxidative Stress And Inflammatory Responses[J]. Molecular Medicine Reports, 2017, 16(4): 5219-5224.
    Sample Type: Cell culture supernatant
  80. Publication: Wang C M, Zhang T, Wang Y H, et al. The Shortening Telomere Length Of T Lymphocytes Maybe Associated With HyperFunction In Servere Aplastic Anemia[J]. Molecular Medicine Reports, 2018, 17: 1015-1021.
  81. Publication: Lu A Q, Wang Z R, Wang S C. Role Of miR-589-3p In Human Lumbar Disc Degeneration And Its Potential Mechanism[J]. Experimental and Therapeutic Medicine, 2018, 15: 1616-1621.
    Sample Type: Cell culture supernatant
  82. Publication: Cao Z, Chen L. Inhibition Of miR27a Suppresses The Inflammatory Response Via The p38/MAPK Pathway In Intervertebral Disc Cells[J]. Experimental and Therapeutic Medicine, 2017, 14(5): 4572-4578.
    Sample Type: Cell culture supernatant
  83. Publication: Li D, Li B, Yang L, et al. Human cytomegalovirus infection is correlated with atherosclerotic plaque vulnerability in carotid artery[J]. The Journal of Gene Medicine, 2020: e3236.
    Sample Type: Serum
  84. Publication: Al-Hilali H A, Abduljaleel A K. The role of TNF and Resistin Gene+ 299 (G/A) polymorphism in the development of insulin resistance in non-obese Type 2 Diabetes Mellitus Iraqi patients[J]. Int J Curr Microbiol App Sci, 2015, 4(10): 475-486.
    Sample Type: Serum
  85. Publication: Abdulazeem L, Al-Dahmoshi H O M, Ali J A. Microbial Index and TNF-α, IL-4, CCL17 Level Among Burn Wound Infections in Hilla City, Iraq[J]. Biomedical and Pharmacology Journal, 2016, 9(3): 1095-1100.
    Sample Type: Serum
  86. Publication: Rudnicka K, Niewiadomska A, Skibińska P, et al. The activation, ROS induction and cytokine secretion in human monocytes exposed to H. pylori lipopolysaccharide depend on the availability of TLR4/2 and TLR2/6 receptor complexes[C]//European Microscopy Congress 2016: Proceedings. Weinheim, Germany: Wiley‐VCH Verlag GmbH & Co. KGaA, 2016: 235-237.
    Sample Type: Cell culture supernatant
  87. Publication: Iduh P D, Adias T C, Eze E M. Evaluation of Cytokines Response in Plasmodium falciparum Infection among Adolescents in Port Harcourt, Nigeria[J]. JMSCR,2018.
    Sample Type: Plasma
  88. Publication: Al-Hakeim H, Almulla A, Maes M. The neuro-immune fingerprint of major neuro-cognitive psychosis or deficit schizophrenia: a supervised machine learning study[J]. Preprint, 2019.
    Sample Type: Serum
  89. Publication: Al-Saadi M A K, Al-Hilli N, Sheriff N A. Influence of TLR4 Polymorphisms on Susceptibility to UTI Caused by Gram Negative Bacteria in Pregnant Women[J]. Indian Journal of Forensic Medicine & Toxicology, 2019, 13(4): 1140-1144.
    Sample Type: Serum,Urine
  90. Publication: Hardiany N S, Yohana Y, Wanandi S I. TNFR, TRAF2, NF-κB mRNA Levels of Glioblastoma Multiforme Cells Treated by Conditioned Medium of Umbilical Cord-derived Mesenchymal Stem Cells[J]. The Indonesian Biomedical Journal, 2019, 11(2).
    Sample Type: Cell lysate
  91. Publication: Noor Aziz Jasim, Kareem Hamed Ghali, et al. Levels of IgG-ADV and Tnfα in Serum of Celiac Disease Patients[J].Scholars Journal of Applied Medical Sciences, 2019.
    Sample Type: Serum
  92. Publication: Adediji I O, Oluremi A S, Adelakun A A, et al. Correlation of Small Dense Low Density Lipoprotein, Tumour Necrosis Factor - alpha with Liver Enzymes in Chronic Hepatitis B Patients[J]. International Journal of Tropical Disease & Health, 2019: 1-8.
    Sample Type: Serum
  93. Publication: Al-Saadi M A K, Al-Hilli N M S, Sheriff N A. Association of TLR4 Polymorphisms with Susceptibility to Urinary Tract Infection Caused by Gram Positive Bacteria in Pregnant Women[J]. Indian Journal of Public Health, 2020, 11(02): 1941.
    Sample Type: Serum
  94. Publication: AAli J, ARAl-KAFAJI Z, HAl-JOTHERY A. Estimation of IL-1βand TNF Concentration in Children Suffering from Febrile Convulsion[J]. The Journal of Research on the Lepidoptera, 2020, 51(2):225-231.
    Sample Type: Serum
  95. Publication: Zeng J, Yang L, Zheng Y. Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma[J]. Preprint, 2020.
    Sample Type: Cell culture supernatant
  96. Publication: Sheng C, Wang Z, Yao C, et al. CALML6 Controls TAK1 Ubiquitination and Confers Protection against Acute Inflammation[J]. The Journal of Immunology, 2020, 204(11): 3008-3018.
    Sample Type: Cell culture supernatant
  97. Publication: Jassim S D, Witwit L J, Abood F M. High Levels of Tumor Necrosis Factor-Alpha and Interferon-Gamma in Patients with Gingival Recession[J]. Medico Legal Update, 2020, 20(1): 804-808.
    Sample Type: Serum
  98. Publication: Al-Ubaidi G T, Abbas A A, Taha A A, et al. Evaluation of Synergistic Effect of Cytosine-Phosphodiesterbond-Guanosin-Oligodeoxynucleotide 7909, and Protamine on Transfection Process Mediated by Calcium Phosphate Nanoparticles[J]. International Journal of Drug Delivery Technology, 2020, 10(1): 52-59.
    Sample Type: Cell culture supernatant
  99. Publication: Almulla A F, Moustafa S R, Al-Dujaili A H, et al. Lowered Serum Cesium Levels in Schizophrenia: Association with Immune-Inflammatory Biomarkers and Cognitive Impairments[J]. 2020.
    Sample Type: Serum
  100. Publication: Onyemelukwe O U, Maiha B B. Hyperhomocysteinemia and folate levels in normal healthy Nigerians living in Zaria: Subanalysis of ABU homocysteine cross-sectional survey[J]. Annals of African Medicine, 2019, 18(3): 143.
    Sample Type: Plasma
  101. Publication: Huang Y, Zhou F, Xiao Y, et al. TLR7 mediates increased vulnerability to ischemic acute kidney injury in diabetes[J]. Revista da Associa??o Médica Brasileira, 2019, 65(8): 1067-1073.
    Sample Type: Cell culture supernatant
  102. Publication: Sun H B, Li Y, Liu X B, et al. Impact of an Early Oral Feeding Protocol on Inflammatory Cytokine Changes After Esophagectomy[J]. The Annals of thoracic surgery, 2019, 107(3): 912-920.
    Sample Type: Serum
  103. Publication: Al-Jebouri M M, Al-Mahmood B Y R. Estimation of Cytokines Involved in Acute-Phase Wound Infection with Reference to Residence Time of Patients in Hospitals[J]. 2019.
    Sample Type: Serum
  104. Publication: Xiao Y, Yang Y, Cao H, et al. Resveratrol protects human renal proximal tubular cells from high glucose and hypoxia/reoxygenation induced injury via inhibiting p38-MAPK and thioredoxin-interacting protein (TXNIP) pathways[J]. Int J Clin Exp Med, 2019, 12(3): 2243-2254.
    Sample Type: Cell culture supernatant
  105. Publication: Mohammed O K, Alabbassi M G, Aldujaili A A, et al. Effect of General and Spinal Anesthesia on Some Anti-inflammatory cytokines and cell adhesion molecules of Iraqi Women Undergo Cesarean Section: Comparative Study[J]. Journal of Pharmaceutical Sciences and Research, 2018, 10(11): 2846-2849.
    Sample Type: Serum
  106. Publication: Shen J, Yao R, Jing M, et al. Sinomenine Regulates Inflammatory Response and Oxidative Stress via Nuclear Factor kappa B (NF-kB) and NF-E2-Related Factor 2 (Nrf2) Signaling Pathways in Ankle Fractures in Children[J]. Medical science monitor: international medical journal of experimental and clinical research, 2018, 24: 6649.
    Sample Type: Serum
  107. Publication: AZIZ T A. EFFECTS OF GINKGO BILOBA EXTRACT (EGb761) AS ADD-ON WITH METFORMIN IN THE TREATMENT OF PATIENTS WITH METABOLIC SYNDROME AND TYPE 2 DIABETES MELLITUS IN SULAIMANI[D]. University of Sulaimani, 2018.
    Sample Type: Serum
  108. Publication: Jasim N A, Kamal Y M, Mohammed M M, et al. 14. Evaluation of the Anti-Inflammatory Effect of Telmisartan As an Adjuvant Therapy to NSAID in The Management of Knee Osteoarthritis; A Clinical Prospective Study[J]. Iraqi JMS 2018; Vol. 16(1): 100-110.
    Sample Type: Serum
  109. Publication: Al-Assadi N A M, Al-Qaysi S A. Tumor Necrosis Factor Alpha Synergetic with Insulin Resistance Potentially Contributes to the Development of Polycystic Ovary Syndrome[J]. Medical Journal of Babylon, 2017, 14(4): 732-737.
    Sample Type: Serum
  110. Publication: Saleh H H, Abdulridha M K, Najeb A A. Potential Benefit of Melatonin as an Adjuvant Therapy in a Sample of Iraqi Patients with Crohn’s Disease: A New Study[J]. ALMUSTANSIRIYAH Journal of PHARMACEUTICAL SCIENCES (AJPS), 2018, 17(2): 9-9.
    Sample Type: Serum
  111. Publication: Nada S Z, Hatef Z S, Alkutubi H M, et al. Serum Tnfα Levels Are Inversely Associated With Insulin Sensitivity in Diabetic Retinopathy Patients[J]. Kerbala Jorunal of Medicine, 2017, 10(3): 3857-3862.
    Sample Type: Serum
  112. Publication: Zhou P, Liu H, Wu Y, et al. Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response[J]. Medical Science Monitor: International Medical Journal of Experimental And Clinical Research, 2018, 24: 4379.
    Sample Type: Serum
  113. Publication: Wang F, Li X, Huang L, et al. High-Mobility Group Protein Box 1 is Upregulated in Children with Henoch-Schonlein Purpura[J]. Pediatric Allergy, Immunology, and Pulmonology, 2018, 31(2): 66-72.
    Sample Type: Serum
  114. Publication: Giusti I, Di Francesco M D, D'Ascenzo S, et al. Leukocyte Depletion Does Not Affect The In Vitro Healing Ability Of Platelet Rich Plasma[J]. Experimental and Therapeutic Medicine, 2018, 15(4): 4029-4038.
  115. Publication: Hatef Z S, Nada S Z, Jasim A M. Adiponectin may be used as a marker in prediction of diabetic retinopathy patients[J]. Iraq Medical Journal, 2018, 2(1): 20-23.
    Sample Type: Serum
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