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For research use only. Order now, ship in 3 days
Product Introduction | Insulin (INS) is a double-stranded (α, β) polypeptide hormone secreted by islet beta cells. It is the only known hormone in the body that reduces blood sugar and simultaneously promotes glycogen, fat, and protein synthesis. Transferrin is an indispensable component in body fluids. It is not only involved in iron transport and metabolism, respiration, cell proliferation and immune system regulation, but also can regulate iron ion balance and energy balance. And it has antibacterial and bactericidal function. Sodium Selenite is a cofactor that provides glutathione peroxidase and other proteins and is used as an antioxidant in culture. Originally used in combination with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solutions are now commonly used in combination with other basal medium to support multiple cell types in the presence of 2-4% FBS. ITS is formulated with Earle balanced salt solution (EBSS). Pricella offers three different ITS: ITS-G for general use; ITS-A for adherent cell culture; and ITS-X for cell types requiring aminoethanol, such as bronchial epithelial cells. |
Concentration | 100 × |
Format | Liquid |
Bacterial Detection | Negative |
Fungal Detection | Negative |
Mycoplasmal Detection | Negative |
pH | 7.8-8.3 |
Animal Origin Ingredient | Without |
Endotoxin Level | <3 EU/mL |
Storage | This product can be stored at 2-8℃ for 18 months. |
Shipping | Ice bag |
Expiration Date | 18 months |
Matters Need Attention | 1. This product is for research use only. 2. This product is sterilized by 0.1 μm filtration. 3. It is necessary to pay attention to the aseptic operation and avoid the contamination. 4. This product is 100 × concentrated solution. Please dilute it to to the desired concentration before use. |
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Q1:What solvent is used for insulin - transferrin - selenium (ITS-G) configuration?
ITS supplements are used to reduce the amount of fetal bovine serum (FBS) needed for routine maintenance and low-density attachment of many cell types. Originally, they are used in combination with RPMI 1640 and MEM. Now, they are commonly used in combination with other basal media to support multiple cell types in the presence of 2 to 4% FBS. Earle’s Balanced Salt Solution (EBSS)can be used for ITS configuration.