Ishikawa Cell Line-Laboratory Biological Research Reagents Manufacturer for Life Science - Elabscience
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Ishikawa Cell Line
Cat Number: EP-CL-0283
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Lead Time: Order now, ship in 3 days
Origin and General Characteristics
||Ishikawa Cell Line
||Homo sapiens, Human
Culture Conditions and Handling
|Complete Growth Medium
||DMEM +10% FBS +1% P/S
(Ishikawa Cell Line complete medium (EP-ML-0283) is recommended.)
||Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
||Every 2 to 3 days
||Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
||Atmosphere: Air, 95%; CO2, 5%
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