Ishikawa Cell Line-Laboratory Biological Research Reagents Manufacturer for Life Science - Elabscience


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Ishikawa Cell Line

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Cat Number:  EP-CL-0283
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Origin and General Characteristics

Cell Name Ishikawa Cell Line
Organism Homo sapiens, Human
Tissue Uterus, endometrium
Morphology Epithelial
Growth Properties Adherent
Biosafety Level 1

Culture Conditions and Handling

Complete Growth Medium DMEM +10% FBS +1% P/S
(Ishikawa Cell Line complete medium (EP-ML-0283) is recommended.)
Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio 1:2-1:4
Medium Renewal Every 2 to 3 days
Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

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Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

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