MTT Assay Kit (Cell Proliferation)[341]

Introduction

Elabscience^® MTT Assay Kit is a rapid and highly sensitive kit widely used in cell proliferation and cytotoxicity detection.

Detection Principle

MTT is 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide which can be reduced by some dehydrogenases in mitochondria to form a crystalline dark purple product formazan. Formazan can be completely dissolved by DMSO and its absorbance is near 570 nm wavelength. The more and faster the cells proliferate, the darker the color is, and the more cytotoxic, the lighter the color is. For the same cells, there is a linear relationship between the depth darkness of color and the number of cells.

Components

Reagents Not Included

DMSO, PBS.

Instructions

MTT(5 ×)[ E-CK-A341A] is concentrated, please diluted with MTT Substrate to 1 × MTT working solution before use.

For example: take 100 μL MTT(5 ×)[ E-CK-A341A], add to 400 μL MTT Substrate[E-CK-A341B], and the mixture is 1 × MTT working solution.

Storage

Store at 2~8°C for half a year, MTT (5 ×)[ E-CK-A341A] should be stored in dark.

Cautions

1.  For maximal assay performance, this reagent should be used within 6 months. Avoid freeze / thaw cycles.

2.  Suspension cells must be centrifuged and absorb supernatant before adding DMSO.

3.  The format of formazan is depend on living cells and incubate time, Mix the cell suspension before adding to the wells to avoid cell precipitation leading to different cell number in each well. The OD value may change rapidly with time when the cell number is large.

4.  MTT is yellow and needs to be stored in dark. Long-term illumination will lead to failure. Do not use when the color turns grey green. MTT may solidify at 4°C or lower temperature. It can be incubated in water bath at 20~25°C for a short time until it is completely melted before use.

5.  For your safety and health, please wear the lab coat and disposable gloves before the experiments.

Citations