QuicKey Pro Rabbit E2(Estradiol) ELISA Kit
E-OSEL-RB0001
Size: | Price: | $520 | |
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QuicKey Pro Series
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
Product Details
Properties
Assay type | Competitive-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 5.65 pg/mL |
Sample type &Sample volume | serum and plasma; 50μL |
Specificity | This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum and plasma. |
Test Principle
Kit components & Storage
Item | Specifications | Storage |
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Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
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Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
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Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
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Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Technical Data
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level E2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level E2 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 50.21 | 96.93 | 495.65 | 46.35 | 90.65 | 490.39 |
Standard deviation | 3.06 | 4.85 | 23.30 | 2.82 | 4.56 | 24.23 |
CV (%) | 6.10 | 5.00 | 4.70 | 6.08 | 5.03 | 4.94 |
Recovery
The recovery of E2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 85-97 | 90 |
EDTA plasma(n=8) | 90-102 | 97 |
Linearity
Samples were spiked with high concentrations of Rabbit E2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Target Information
Synonyms | Oestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol |
Research Area | Cell biology, Metabolism |