Rabbit IL-1β(Interleukin 1 Beta) ELISA Kit (E-EL-RB0013)
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Sensitivity | 9.38 pg/mL |
Detection Range | 15.63-1000 pg/mL |
Sample Volume | 100 μL |
Total Assay Time | 3 h 30 min |
Reacitivity | Rabbit |
Specificity | This kit recognizes Rabbit IL-1β in samples. No significant cross-reactivity or interference between Rabbit IL-1β and analogues was observed |
Recovery | 80%-120% |
Sample Type | Serum, plasma and other biological fluids |
Detection Method | Colorimetric method, ELISA, Sandwich |
Assay Type | Sandwich-ELISA |
Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
Storage | 2-8℃ |
Expiration Date | 12 months |
Uniport ID | P14628 |
Research Area | Cancer, Cardiovascular, Metabolism, Immunology |
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1 Results
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1 Results
- Seawater immersion exacerbates the pathological changes caused by incisive corneal injury in rabbit eyes
IF:3.616
Journal:Annals of Translational Medicine(2022)
DOI:10.21037/atm-22-2198Reactivity:Rabbit
Sample Type:aqueous humor
- CRISPR/Cas9-mediated knockout of APOC3 stabilizes plasma lipids and inhibits atherosclerosis in rabbits
IF:3.876
Journal:Lipids in Health and Disease(2021)
DOI:10.1186/s12944-021-01605-7Reactivity:Rabbit
Sample Type:plasma
- Anti-osteoarthritis effect of a combination treatment with human adipose tissue-derived mesenchymal stem cells and thrombospondin 2 in rabbits
IF:3.534
Journal:World Journal of Stem Cells(2019)
DOI:10.4252/wjsc.v11.i12.1115Reactivity:Rabbit
Sample Type:Synovia
- Acupuncture attenuates renal interstitial fibrosis via the TGF?β/Smad pathway
IF:1.851
Journal:Molecular Medicine Reports(2019)
DOI:10.3892/mmr.2019.10470Reactivity:Rabbit
Sample Type:blood
- The Efficiency of Cyclosporine A-Eluting Contact Lenses for the Treatment of Dry Eye
IF:1.672
Journal:CURRENT EYE RESEARCH(2019)
DOI:10.1080/02713683.2018.1563702Reactivity:Rabbit
Sample Type:Tissue homogenate
- Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7
IF:6.183
Journal:EBioMedicine(2018)
DOI:10.1016/j.ebiom.2018.09.020Reactivity:Rabbit
Sample Type:Plasma
- Establishment of a New Zealand White Rabbit Model for Lethal Toxin (LT) Challenge and Efficacy of Monoclonal Antibody 5E11 in the LT-Challenged Rabbit Model
IF:3.273
Journal:Toxins(2018)
DOI:10.3390/toxins10070289Reactivity:Rabbit
Sample Type:Serum
- Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1–7) upregulation
IF:2.759
Journal:BIOMEDICINE & PHARMACOTHERAPY(2017)
DOI:10.1016/j.biopha.2017.11.151Reactivity:Rabbit
Sample Type:Cell culture supernatant,Serum
- Effects of local lipopolysaccharide administration on the expression of Toll-like receptor 4 and pro-inflammatory cytokines in uterus and oviduct of rabbit does
IF:1.986
Journal:THERIOGENOLOGY(2017)
DOI:10.1016/j.theriogenology.2017.10.046Reactivity:Rabbit
Sample Type:Plasma
- Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits
IF:1.692
Journal:Molecular Medicine Reports(2017)
DOI:10.3892/mmr.2017.8304Reactivity:Rabbit
Sample Type:joint fluid
Q1:Why is it necessary to add a protease inhibitor in tissue sample preparation during an Elisa experiment? Will it affect the detection significantly if there is no protease inhibitor?
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
Q2:My sample volume is small. Can I reduce some reagents proportionally?
No, the detection system of our kits requires strict adherence to the specified sample volume to ensure accurate detection. If the sample volume is insufficient, consider diluting appropriately, but first conduct a pre-experiment to confirm the suitable dilution factor.
Q3:I need to measure corticosterone and testosterone in hair samples. Are there any suggested sample extraction methods?
Hair sample preparation method: Wash hair samples with methanol by adding 5 mL of HPLC-grade methanol to each sample, rotating for 3 minutes, then decanting excess methanol and rinsing hair twice. After washing, place the hair samples on aluminum foil, dry for 3 days in a protective cap. Weigh the dried hair samples and transfer them to 2ml polypropylene tubes containing stainless steel grinding beads. Place the tubes containing hair and beads in a bead beater, grind each sample for 2 minutes to produce powder. After grinding, add 1.5 mL of methanol to the tubes containing hair powder. Rotate samples slowly for 24 hours to extract steroids. Centrifuge at 10000 g for 4 minutes, transfer 0.6 mL of methanol supernatant containing steroids to new 1.5 mL microcentrifuge tubes. Dry the samples in a protective hood for 2-3 days to evaporate the methanol. Dilute the dried extract with 0.4 mL dilution buffer from the kit for detection.
Q4:Do your ELISA kits have the enzyme that catalyzes the substrate reaction is not HRP? My sample is red blood cell lysate, and HRP will interfere with hemoglobin. Or is there a way to remove Hb?
The detection antibodies in our ELISA kits are all HRP-based. Currently, there are no kits available for testing red blood cell lysate. For small amounts of hemoglobin, consider increasing the number of washing steps by 1-2 during the first plate washing step to reduce interference. However, besides hemoglobin, substances such as endogenous HRP enzymes released during the process of red blood cell lysis can also catalyze the substrate, so we still do not recommend testing with such samples.
Q5:Do you have ELISA kits for plant or microbial samples?
Currently, our ELISA kits are only suitable for animal samples, and we do not have kits for testing plant/microbial samples.
Q6:Do you have a suggested preparation method for vaginal discharge samples?
After the collection with the swab, elute with 500μl PBS, centrifuge at 5000×g for 10 minutes (if the sample remains turbid, try centrifuging at 10000×g), then collect the supernatant for testing.
Q7:Can ELISA kits be used on automated analyzers?
Our ELISA kits have not been validated for compatibility with automated analyzers. The provided instructions are for manual operation. Customers may validate them on their own if needed.
Q8:Can a shaker or a water bath be used during the incubation stage in ELISA experiments?
To ensure temperature fluctuations during incubation stay within 1°C, it's recommended to use an incubator. If an incubator is not available, a water bath may be used, but precautions should be taken to avoid contaminating the plate with water. Using a shaker, especially one with vibration, for incubation is not recommended.