For research use only.
Verified Samples | Verified Samples in WB: Mouse brain, Mouse hippocampus, Mouse cerebellum, Rat brain, Rat hippocampus, Rat cerebellum Verified Samples in IHC: Mouse brain, Rat brain |
Dilution | WB 1:500-1:2000, IHC 1:300-1:800 |
Isotype | IgG |
Host | Rabbit |
Reactivity | Mouse, Rat |
Applications | WB, IHC |
Clonality | Polyclonal |
Immunogen | KLH conjugated Synthetic peptide corresponding to NeuN |
Abbre | RBFOX3 |
Synonyms | FLJ56884, FLJ58356, FOX3NeuN, Fox-1 homolog C, Fox3, HRNBP3, NEUN, RFOX3, RNA binding protein, RNA binding protein fox-1 homolog 3, Rbfox3, fox 1 homolog (C. elegans) 3, fox1 homolog C, hexaribonucleotide binding protein 3, neuronal nuclei |
Swissprot | |
Calculated MW | 46-48 kDa |
Observed MW | 46-48 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm, Nucleus. |
Concentration | 1 mg/mL |
Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
Purification Method | Affinity purification |
Research Areas | Cancer, Epigenetics and Nuclear Signaling, Neuroscience, Tags and Cell Markers |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism. |
Other Clones
1 Results
Other Formats
1 Results
Unconjugated