Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag)(Active)-Laboratory Biological Research Reagents Manufacturer for Life Science - Elabscience

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Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag)(Active)

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Price:   $1084
Cat Number:  PKSR030500
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Overview

Synonyms 2019-nCov S protein; 2019-nCoV Spike glycoprotein; 2019-nCoV S glycoprotein
Species Virus
Expression_host HEK293 Cells
Sequence Arg319-Phe541
Accession YP_009724390.1
Mol_Mass 51.5 kDa
Tag C-mFc
Bio_Activity 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) (Cat#PKSR030508) at 2μg/mL (100μL/well) can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) (Cat#PKSR030500), the EC50 of PKSR030500 is 15-50 ng/mL. 2. Loaded Human ACE2 Protein (His tag) (Cat#PKSR030508) on SA Biosensor, can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) (Cat#PKSR030500) with an affinity constant of 0.562 nM as determined by Octet RED System.

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per µg as determined by the LAL method.
Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation LyopHilized from sterile PBS, pH 7.4.
Reconstitution Please refer to the printed manual for detailed information.

Background

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

SDS-PAGE

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