Reduced Glutathione (GSH) Colorimetric Assay Kit (E-BC-K030-M)
For research use only. Order now, ship in 3 days
Detection Principle | Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly. |
Synonyms | GSH |
Sample Type | Serum,Plasma,Cell culture supernatant,Tissue,Cell |
Detection Method | Colorimetric method |
Detection Instrument | Microplate reader (405-414 nm) |
Research Area | Oxidative Stress, Cuproptosis, Ferroptosis |
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
Storage | This product can be stored at 2-8°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 2 μmol/L |
Detection Range | 2-100 μmol/L |
Precision | inter-assay CV: 3.2% | intra-assay CV: 1.9% |
Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
Human serum | 1 |
Human plasma | 1 |
10% Mouse brain tissue homogenization | 1 |
10% Mouse liver tissue homogenization | 1 |
Hela cell homogenization (0.999 mgprot/mL) | 1 |
Rat serum | 1 |
Rat plasma | 1 |
Mouse serum | 1 |
10% Carrot tissue homogenization | 1 |
293T supernatant | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
1 Results
Other Formats
1 Results
- Small extracellular vesicles delivering lncRNA WAC-AS1 aggravate renal allograft ischemia‒reperfusion injury by inducing ferroptosis propagation
IF:12.400
Journal:CELL DEATH AND DIFFERENTIATION(2023)
DOI:10.1038/s41418-023-01198-x - 17β-oestradiol inhibits ferroptosis in the hippocampus by upregulating DHODH and further improves memory decline after ovariectomy
IF:11.400
Journal:Redox Biology(2023)
DOI:10.1016/j.redox.2023.102708Reactivity:Rat
Sample Type:hippocampal tissue
- Shank3 ameliorates neuronal injury after cerebral ischemia/reperfusion via inhibiting oxidative stress and inflammation
IF:11.400
Journal:Redox Biology(2023)
DOI:10.1016/j.redox.2023.102983Reactivity:Mouse
Sample Type:brain tissue
- Identification of ZIP8-induced ferroptosis as a major type of cell death in monocytes under sepsis conditions
IF:11.400
Journal:Redox Biology(2023)
DOI:10.1016/j.redox.2023.102985Reactivity:Mouse
- Multifunctional nanoadjuvant-driven microenvironment modulation for enhanced photothermal immunotherapy in breast cancer
IF:10.800
Journal:JOURNAL OF CONTROLLED RELEASE(2023)
DOI:10.1016/j.jconrel.2023.08.046Reactivity:Mouse
Sample Type:4T1 cell-xenograft
- Polystyrene nanoplastics lead to ferroptosis in the lungs
IF:10.700
Journal:Journal of Advanced Research(2023)
DOI:10.1016/j.jare.2023.03.003Reactivity:Human
- Nano-selenium enhances melon resistance to Podosphaera xanthii by enhancing the antioxidant capacity and promoting alterations in the polyamine, phenylpropanoid and hormone signaling pathways
IF:10.200
Journal:JOURNAL OF NANOBIOTECHNOLOGY(2023)
DOI:10.1186/s12951-023-02148-yReactivity:Melon
Sample Type:leaves
- Extracellular vesicle-derived silk fibroin nanoparticles loaded with MFGE8 accelerate skin ulcer healing by targeting the vascular endothelial cells
IF:10.200
Journal:JOURNAL OF NANOBIOTECHNOLOGY(2023)
DOI:10.1186/s12951-023-02185-7Reactivity:Rat
- A Redox Modulatory Sod Mimetic Nanozyme Prevents The Formation of Cytotoxic Peroxynitrite And Improves Nitric Oxide Bioavailability in Human Endothelial Cells
IF:10.000
Journal:Advanced Healthcare Materials(2023)
DOI:10.1002/adhm.202300621Reactivity:Mouse
Sample Type:serum
- Multi-Enzyme Cascade-Triggered Nitric Oxide Release Nanoplatform Combined with Chemo Starvation-like Therapy for Multidrug-Resistant Cancers
IF:9.500
Journal:ACS Applied Materials & Interfaces(2023)
DOI:10.1021/acsami.3c05337Reactivity:Mouse