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Reduced Glutathione (GSH) Colorimetric Assay Kit (E-BC-K030-M)

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96T $ 150.00
48T $ 120.00
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For research use only. Order now, ship in 3 days

Detection Principle Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.
Synonyms GSH
Sample Type Serum,Plasma,Cell culture supernatant,Tissue,Cell
Detection Method Colorimetric method
Detection Instrument Microplate reader (405-414 nm)
Research Area Oxidative Stress,  Cuproptosis,  Ferroptosis
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 2 μmol/L
Detection Range 2-100 μmol/L
Precision inter-assay CV: 3.2% | intra-assay CV: 1.9%
Assay Time 25 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample TypeDilution Factor
Human serum 1
Human plasma 1
10% Mouse brain tissue homogenization 1
10% Mouse liver tissue homogenization 1
Hela cell homogenization (0.999 mgprot/mL) 1
Rat serum 1
Rat plasma 1
Mouse serum 1
10% Carrot tissue homogenization 1
293T supernatant 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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