SK-N-AS cell line-Laboratory Biological Research Reagents Manufacturer for Life Science - Elabscience

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SK-N-AS cell line

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Cat Number:  EP-CL-0621
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Origin and General Characteristics

Cell Name SK-N-AS cell line
Organism Homo sapiens, human
Age Female,6 years
Tissue Brain; derived from metastatic site: bone marrow
Morphology Epithelial
Growth Properties Adherent
Descriptions This cell line was derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.
Biosafety Level 1

Culture Conditions and Handling

Complete Growth Medium DMEM+10% FBS+1% P/S
(SK-N-AS cell line complete medium (EP-ML-0621) is recommended.)
Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio 1:2-1:5
Medium Renewal Every 2 to 3 days
Cryopreservation Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

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Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

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