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TUNEL In Situ Apoptosis Kit (HRP-DAB Method) (E-CK-A331)

  • TUNEL In Situ Apoptosis Kit (HRP-DAB Method) Operation Guide
All Size Price Qty
100Assays $ 780.00
50Assays $ 480.00
20Assays $ 280.00
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For research use only.

Detection Principle When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.
Detection Method Colorimetric method
Sample Type Paraffin section;frozen section;cell slide
Assay Time 3 hours
Detection Instrument Light microscope
Other Reagents Required 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),Xylene,Ethanol,DNase I,PBS(E-BC-R187),ddH2O,Hematoxylin(E-IR-R120),Neutral gum(E-IR-R118)
Storage This product can be stored at -20°C for 12 months with shading light.
Expiration date 12 months
Shipping Ice bag
Cat.No. Product Name Sizes
E-CK-A211 Annexin V-FITC/PI Apoptosis Kit 200Assays , 100Assays , 50Assays , 20Assays
E-CK-A301 Mitochondrial Membrane Potential Assay Kit (with JC-1) 100Assays , 50Assays , 20Assays
E-CK-A320 One-step TUNEL In Situ Apoptosis Kit (Green, FITC) 100Assays , 50Assays , 20Assays
E-CK-A362 Enhanced Cell Counting Kit 8 (WST-8/CCK8) 500Tests , 100Tests
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