Added to cart successfully !

VERO C1008 [Vero E6] cell line

- +
Price:   Inquire
Cat Number:  EP-CL-0491
Lead Time:   3-5 days
Add to cart

Origin and General Characteristics

Cell Name VERO C1008 [Vero E6] cell line
Organism Cercopithecus aethiops
Age adult
Tissue Normal Kidney
Morphology Epithelial
Growth Properties Adherent
Descriptions This cell line was established in 1979, cloned from VERO 76 into microtiter plates by using the dilution method .
Biosafety Level 1

Culture Conditions and Handling

Complete Growth Medium DMEM+10% FBS+1% P/S
Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio 1: 2-1: 4
Medium Renewal 2-3 times per week
Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5%
Temperature: 37℃

Related Resources

Recommendations for handling of cryopreserved cells

If you have any question about the product, please feel free to contact us or leave a message