VERO C1008 [Vero E6] cell line-Laboratory Biological Research Reagents Manufacturer for Life Science - Elabscience


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VERO C1008 [Vero E6] cell line

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Cat Number:  EP-CL-0491
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Origin and General Characteristics

Cell Name VERO C1008 [Vero E6] cell line
Organism Cercopithecus aethiops
Age adult
Tissue Normal Kidney
Morphology Epithelial
Growth Properties Adherent
Descriptions This cell line was established in 1979, cloned from VERO 76 into microtiter plates by using the dilution method .
Biosafety Level 1

Culture Conditions and Handling

Complete Growth Medium MEM(EP-CM-L0191)+10% FBS+1% P/S(EP-CM-L0048)
(VERO C1008 [Vero E6] cell line complete medium (EP-ML-0491) is recommended.)
Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio 1: 2-1: 4
Medium Renewal 2-3 times per week
Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

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EP-CM-L0048 Penicillin-Streptomycin Solution (100×)
EP-CM-L0006 Phosphate Buffer (PBS)
EP-CM-L0060 200mM L-Alanyl-L-Glutamine Solution (100×)
EP-CM-L0043 0.25% Trypsin Solution

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

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