This cell line was established in 1979, cloned from VERO 76 into microtiter plates by using the dilution method .
Culture Conditions and Handling
Complete Growth Medium
MEM(EP-CM-L0191)+10% FBS+1% P/S(EP-CM-L0048) (VERO C1008 [Vero E6] cell line complete medium (EP-ML-0491) is recommended.)
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.