APC Anti-Human CD25 Antibody[BC96] E-AB-F1194E
Host: Mouse
Applications: FCM
Isotype: Mouse IgG1, κ
Filter:
FCM
Human peripheral blood lymphocytes are stained with FITC Anti-Human CD4 Antibody and APC Anti-Human CD25 Antibody.
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| Price: | $40 |
Datasheet
Antigen Details
| Alternate Names | Interleukin-2 receptor subunit alpha,IL2RA,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,p55 |
| Swissprot | P01589 |
| References |
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681. |
Product Details
| Form | Liquid |
| Clone No. | BC96(See Other Available Formats) |
| Host | Mouse |
| Isotype | Mouse IgG1, κ |
| Isotype Control | APC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792E] |
| Reactivity | Human |
| Application | FCM (See the Application Description) |
| Storage Buffer | PBS with 0.05% Proclin300, 1% BSA |
| Recommended Use | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
| Shipping | Biological ice pack at 4℃ |
| Stability & Storage | Keep as concentrated solution. Store at 2~8℃ and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
| Conjugation | APC |
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Background
| CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex. |
Protocols & Troubleshootings
| Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
| Troubleshooting |
Alternative Formats
| Cat.No. | Product Name | Sizes |
| E-AB-F1194C | FITC Anti-Human CD25 Antibody[BC96] E-AB-F1194C | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1194D | PE Anti-Human CD25 Antibody[BC96] E-AB-F1194D | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1194E | APC Anti-Human CD25 Antibody[BC96] E-AB-F1194E | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1194G | PE/Cyanine5 Anti-Human CD25 Antibody[BC96] E-AB-F1194G | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1194H | PE/Cyanine7 Anti-Human CD25 Antibody[BC96] E-AB-F1194H | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1194I | PE/Cyanine5.5 Anti-Human CD25 Antibody[BC96] E-AB-F1194I | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1194J | PerCP/Cyanine5.5 Anti-Human CD25 Antibody[BC96] E-AB-F1194J | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1194L | AF488 Anti-Human CD25 Antibody[BC96] E-AB-F1194L | 20 Tests, 50 Tests, 100 Tests, 200 Tests |
| E-AB-F1194M | AF647 Anti-Human CD25 Antibody[BC96] E-AB-F1194M | 20 Tests, 50 Tests, 100 Tests, 200 Tests |