Cell proliferation detection is a basic experimental method to evaluate cell activity, genotoxicity and the effect of antitumor drugs. It generally reflects the growth status and activity of cells by analyzing the changes in the number of dividing cells. Cell activity is an important index to judge whether the cultured cells can grow normally under certain conditions in vitro. The detection of cell activity is an indirect method to detect cell proliferation ability. Cytotoxicity can be measured by changes in a number of indicators, including cell viability, cell proliferation, mitochondrial function, phospholipid deposition/lipid degeneration, DNA damage, and cell cycle.
Elabscience® provides cell proliferation and cytotoxicity/activity kits including EdU, CCK-8 and LDH. Depending on the type of sample and the purpose of your experiment, you can choose the appropriate kit according to your experimental requirements
The most accurate method to measure DNA proliferation is by directly measuring DNA synthesis. The most common method for this uses antibody-based detection of the nucleoside analog bromo-deoxyuridine (BrdU).
EdU (5-ethynyl-2 '-deoxyuridine) ,is an alternative to BrdU,which is a thymidine nucleoside analogue that can be incorporated into replicating DNA molecules instead of thymidine (T) during cell proliferation. In contrast to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. EdU utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.
- Simplicity: No antigen-antibody reaction is required, simple and efficient
- Sensitive: without antibody, the detection dye is only 1/500 of BrdU antibody, which is easy to spread and can be accurately detected even for a single proliferating cell
- Fast: No need to stay overnight, only 2~3 hours to complete the test
- Accurate: without DNA denaturation (acid hydrolysis, pyrolysis, enzymatic hydrolysis, etc.), it can effectively avoid sample damage caused by denaturation
- Compatible: almost no damage to the sample, easier to be labeled with a variety of antibodies or fluorescent proteins at the same time, can simultaneously detect other characteristics of the cell
|Product Name||Cat. No||Dye||Size|
|E-Click EdU Cell Proliferation Flow Cytometry Assay Kit (Green, AF488)||E-CK-A371||AF488||50 Assays/ 200 Assays|
|E-Click EdU Cell Proliferation Flow Cytometry Assay Kit (Red, AF647)||E-CK-A372||AF647||50 Assays/ 200 Assays|
|E-Click EdU Cell Proliferation Imaging Assay Kit (Green, AF488)||E-CK-A376||AF488||50~500 Assays/ 200~2000 Assays|
|E-Click EdU Cell Proliferation Imaging Assay Kit (Red, AF594)||E-CK-A377||AF594||50~500 Assays/ 200~2000 Assays|
|E-Click EdU Cell Proliferation Imaging Assay Kit (Red, AF647)||E-CK-A378||AF647||50~500 Assays/ 200~2000 Assays|
Jurkat cells were treated with 10 μM EdU for 4 h (red);without EdU (blue)E-CK-A376
Hela cells were treated with 10 μM EdU for 2 h proliferate cells (green) and cells were counterstained with DAPI (blue)
Cell Counting Kit-8（CCK-8) is a rapid and highly sensitive kit based on WST-8, which is widely used in the detection of cell activity and cytotoxicity.
Wst-8 is a compound similar to MTT. In the presence of electron carrier, WST-8 can be reduced by dehydrogenase in mitochondria to form water-soluble orange-yellow dark (Formazan) products, and the amount of Formazan produced is proportional to the number of viable cells. The more and faster the cell proliferation, the darker the color is. The amount of viable cells can be calculated indirectly by measuring the absorbance at 450 nm.
Because the CCK-8 solution is very stable and has little cytotoxicity, longer incubation periods, such as 24 to 48 hours, can be performed. The detection sensitivity of the CCK-8 kit is higher than that of any other tetrazolium salt, such as MTT, XTT or MTS.
|Wide linear and perfect reproducibility|
|Low toxicity, simple operation|
|Multiple applications, drug screening, tumor drug sensitivity etc.|
Lactate dehydrogenase (LDH) is a kind of oxidoreductase that only exist in cytoplasm of all kinds of cells, it can be released into supernatant during the process of cell death or damage. The quantitation of cytotoxicity can be measured by measuring the extracellular LDH activity through enzymetic reaction.
LDH catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. Under the action of PMS, electrons are transfered from NADH to WST-8 to produce the yellow formazan dye which has a characteristic absorption peak at 450 nm.
- Simple：Positively correlated with the cell death rate (With greater cytotoxicity, comes greater optical density)
- Higher Sensitivity：Giving higher OD value for the same sample (Comparing with other LDH cytotoxicity assay kits)
- Faster：Much shorter reaction time (As short as 10 min)
- Widely Application：A more general application with micro-plate reader using 450 nm detection
|Product Name||Cat. No||Size|
|Lactate Dehydrogenase (LDH) Cytotoxicity Colorimetric Assay Kit||E-BC-K771-M||96 T|
Lactate Dehtdrogenase (LDH) Cytotoxicity Colorimetric Assay Kit
Sample Type: Cell
Higher Sensitivity for the same sample (Comparing with other LDH cytotoxicity assay kits)Much shorter reaction time (As short as 10 min)