Bacterial Expression

Prokaryotic protein expression system is the most common and economical protein expression method today, and the E. coli expression system is one of the most important and widely used prokaryotic protein expression systems. Elabscience's E. coli protein expression system service starts with our proprietary codon optimization and protein analysis (S-CP-P001), then gene will be synthesised and subcloned (S-CP-P002), there are all kinds of pET series vectors and integrate GST or MBP tags to select according to customer needs, and there is tag removal service (S-CP-P005), during the transformation and small test, multiple expression strains such as BL21 (DE3), T7 Express, Rosseta gami (DE3) plys, Shuffle T7B, BL21 Codon Plus (DE3) are provided (S-CP-P003). In addition, we provide endotoxin removal service (S-CP-P008) to meet your special requirements. You can order each service or any combination of these services according to the project plan.

Submit your project information and requirements and place an order. Our experienced experts will help you with your research and promise to provide you with quality services at a competitive price.

Advantages

Competitive price: Starting from $1,200.

Short period: As short as 5 weeks.

Guaranteed Package: 1~5 mg of purified proteins with a competitive price of $1,980.

High-purity protein protein production: A variety of purification methods combined with protein purification system can customize purity ≥95%.

One-stop service platform: Elabscience^® can take your project directly from gene synthesis to downstream activities including recombinant protein expression, antibody production, and assay development.

E.coli Expression and Purification Service (E.coli System) Details

Cat. No.	Service Name	Timeline	Delivery	Quoted price
S-CP-P001	Analysis of gene and target protein	1-2 days	Analysis of results	Free
S-CP-P002	Gene synthesis and preparation of expression vector	2-3 weeks	Expression vector containing the target gene Target sequencing report Escherichia coli containing expression vector plasmid Related analysis reports	$0.4/bp
S-CP-P003	Transform and optimized expression condition	1-2 weeks	The optimum inducing conditions (temperature, time, concentration of IPTG) Analysis of protein solubility	$460
S-CP-P004	Large scale expression in 1L and tag affinity purification	2-3 weeks	1L bacterial expression, purity≥80% of target protein fused with His tag Related analysis reports	$460（The price of each additional 1L increases $400）
S-CP-P005	Tag removal and subsequent purification	1-2 weeks	1L bacterial expression, purity≥85%, without tag target protein Enzyme digestion test and purification report	$460
S-CP-P006	The secondary purification	1 week	Purity≥90%, total 1-5mg target protein Related analysis reports	$400
S-CP-P007	Final purification	1 week	Purity≥95%, total 1-5mg target protein Related analysis reports	$500
S-CP-P008	Endotoxin removal	1 week	1-5mg target protein, endotoxin < 1Eu /μg	$500