QuicKey Pro Human POSTN/OSF-2(Periostin) ELISA Kit
E-OSEL-H0013
| Size: | Price: | $520 | |
| Qty: |
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QuicKey Pro Series
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
Product Details
Properties
| Assay type | Sandwich-ELISA |
| Format | 96T/48T/24 |
| Assay time | 1.5h |
| Detection range | 3.12-200 ng/mL |
| Sensitivity | 1.52 ng/mL |
| Sample type &Sample volume | serum and plasma; 50μL |
| Specificity | This kit recognizes POSTN/OSF-2 in samples. No significant cross-reactivity or interference between POSTN/OSF-2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Application | This ELISA kit applies to the in vitro quantitative determination of POSTN/OSF-2 concentrations in serum and plasma. |
Test Principle
Kit components & Storage
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
| Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
| Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
| HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
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| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
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| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
| Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
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| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Technical Data
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level POSTN/OSF-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level POSTN/OSF-2 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.99 | 6.27 | 13.47 | 2.17 | 6.59 | 13.68 |
| Standard deviation | 0.13 | 0.32 | 0.62 | 0.12 | 0.35 | 0.42 |
| CV (%) | 6.78 | 5.17 | 4.61 | 5.58 | 5.32 | 3.04 |
Recovery
The recovery of POSTN/OSF-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-103 | 98 |
| EDTA plasma(n=8) | 84-99 | 91 |
Linearity
Samples were spiked with high concentrations of Human POSTN/OSF-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Target Information
| Database Links | SwissProt: Q15063 |
| Synonyms | POSTN/OSF-2 |
| Research Area | Cancer, Cardiovascular, Signal transduction, Tags and Cell Markers |
Assay Procedures
