Ramos [RA 1] Cell Line
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Origin and General Characteristics
| Cell Name | Ramos [RA 1] Cell Line |
| Organism | Homo sapiens, Human |
| Age | 3 years |
| Tissue | Peripheral blood |
| Morphology | Lymphoblast |
| Growth Properties | Suspension |
| Descriptions | The cells are negative for Epstein-Barr virus. Both the parental line and the derivative cell line Ramos. 2G6. 4C10 have about 1500 IL-4 binding sites per cell. The cells are reported to secrete IgM(lambda light chain). |
| Biosafety Level | 1 |
Culture Conditions and Handling
| Complete Growth Medium | RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120) (Ramos [RA 1] Cell Line complete medium (CM-0483) is recommended.) |
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5. |
| Subcultivation Ratio | 1:2 to 1:4 |
| Medium Renewal | Every 2 to 3 days |
| Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃ |
Special Features of the Cell Line
| Tumorigenic | Yes |
| Effects | Yes, forms tumors in nude mice. |
| Receptor Expression | Interleukin 4(IL-4); low affinity IgE(CD23) |
| Antigen Expression | CD23＋ |
| Gene Expression | Immunoglobulin(Surface And Secreted) |
Related Products
| PM150110P | RPMI-1640, powder |
| PM150110 | RPMI-1640 |
| PB180120 | Penicillin-Streptomycin Solution, 100× |
| PB180327 | Phosphate Buffer (PBS) |
| PB180419 | 200 mM L-Alanyl-L-Glutamine Solution, 100× |
Technical Resources
Recommendations for handling of cryopreserved cells
