Preparation of Tissue Samples for Metabolism Assays

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Take 0.02-1g fresh tissue to wash with homogenization medium at 2-8℃ to remove blood cells. Absorb the water with filter paper and weigh. Homogenize at the ratio of the volume of homogenized medium (2-8℃) (mL): the weight of the tissue (g) =9:1, then centrifuge the tissue homogenate for 10 min at 10000 g at 4℃. Take the supernatant to preserve it on ice for detection.