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Proteinase K Lyophilized Powder

Size:  
Qty:
- +
Price:   $350
Cat Number:  E-IR-R109U
Lead Time:   7~10 days

Storage:  2~8°C

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Introduction

Protease K is a kind of high activity protease of subtilisin, which is used to degrade proteins in biological samples. It can be used to digest various proteins, and it can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on.

Enzyme activity, > 30 U/mg. At 37°C, the amount of Protease K that can produce amino acids or polypeptides equivalent to 1 micromol of tyrosine Folin positive in one minute with hemoglobin as the substrate is defined as a unit of protease K activity.

The effective pH range of Protease K is pH4.0~12.5, and the optimal pH range is pH7.5~8.0.

The optimum reaction temperature of protease K is 65°C, but at 65°C or higher, Protease K can also degrade rapidly. It is suggested that the optimum reaction temperature is 50~55°C.


Components



Cat.

Products

1 g

10 g

100 g

Storage

E-IR-R109U

Proteinase K Lyophilized Powder

1 g

10 g

100 g

2~8°C

Manual

 One Copy



Specification parameters

Source

Yeast

Appearance

White loose powder

MW.

29 kDa

CAS NO.

39450-01-6

Purity

95% by SDS-PAGE

E.C

3.4.21.64

Activity

30U/mg

DNase

No

Buffer

10mM Tris-HClpH 7.5,5mM CaCl 2

RNase

No


Usage

Reconstitution. Dissolve the powder with 50% glycerin. It is recommended to keep the solution at 2~8°C.

Use it as soon as possible after opening and please avoid pollution.


Storage

Store at RT or lower temperature.

For long time storage, the lyophilized powder is stable for at least two years at 2~8°C.

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