QuicKey ELISA Operation Guide Video

This ELISA kit uses the Sandwich-ELISA principle.The micro ELISA plate provides in this kit has been pre-coated with specific antibody. Samples (or standards) and biotinylated detection antibody specific are added to the micro ELISA plate wells at the same time. The antigen in the sample (or standard) would combine with the coated antibody, and the biotinylated detection antibody combine with the antigen bound to the coated plate. Free components are washed away. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well , biotin and avidin are specifically combined to form an immune complex. Free components are washed away. The substrate solution is added to each well. Only those wells that contain antigen, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of the antigen. You can calculate the concentration of the antigen in the samples by comparing the OD of the samples to the standard curve.