Cell Intracellular Targets Staining for Flow Cytometry

This is an easy tutorial about cell intracellular targets staining for flow cytometry, this video shows the experiment procedure of flow cytometry such as: Block Fc Receptor, Set Sample and Control, Cell Surface Staining, Fixation and Permeabilization, so that the experimenters can better understand this experiment.

Procedure:

Sample Preparation→Cell Counting→Block Fc Receptor(optional)→Cell Surface Staining→ Fixation and Permeabilization→Cell Intracellular Staining→Detection→Analysis