QuicKey Pro ELISA (Competitive Method) Operation Guide Video
This is a video about QuicKey ProTM ELISA (Competitive Method) Kit.
The micro ELISA plate provided in this kit has been pre-coated with the antigen. Samples (or Standards) and HRP conjugate (enzyme-labeled antibody) are added to the micro ELISA plate wells at the same time. The antigen in samples (or standards) competes with a fixed amount of antigen on the solid phase supporter for sites on the enzyme-labeled antibody to form a complex of the coated antigen-enzyme-labeled antibody. Then the free components are washed away from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is inversely proportional to the concentration of antigen. You can calculate the concentration of antigen in the samples by comparing the OD of the samples to the standard curve.