QuicKey Pro ELISA (Sandwich Method) Operation Guide Video
This is a video about QuicKey ProTM ELISA (Sandwich Method) Kit.
The micro ELISA plate provides in this kit has been pre-coated with antibody. Samples (or Standards) and HRP conjugate (enzyme-labeled antibody) are added to the micro ELISA plate wells at the same time. The antigen in samples (or standards) combines with the coated antibody and enzyme-labeled antibody to form a complex of the coated antibody-antigen- enzyme-labeled antibody. Then the free components are washed away from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm, and the OD value is proportional to the concentration of the antigen. You can calculate the concentration of antigen in the samples by comparing the OD of the samples to the standard curve.