Preparation of Stacking Gel

Components

Sample volume of each component corresponding to different stacking gel volumes

1 mL

2 mL

3 mL

4 mL

5 mL

6 mL

8 mL

10 mL

5% stacking gel

H₂O

0.68

1.4

2.1

2.7

3.4

4.1

5.5

6.8

30% Acrylamide

0.17

0.33

0.5

0.67

0.83

1.0

1.3

1.7

1.0M Tris-HCl(pH 6.8)

0.13

0.25

0.38

0.5

0.63

0.75

1.0

1.25

10% SDS

0.01

0.02

0.03

0.04

0.05

0.06

0.08

0.1

10% (NH₄)₂S₂O₈

0.01

0.02

0.03

0.04

0.05

0.06

0.08

0.1

TEMED

0.001

0.002

0.003

0.004

0.005

0.006

0.008

0.01