QuicKey ELISA^® Kits

QuicKey ELISA^® Kit, is an improvement of the traditional sandwich ELISA Kit, it simplifies the operation process, and only 2 washing steps and 3 incubation steps are required. It shortens the experiment time from 3.5 hours to 2.5 hours without compromising high sensitivity, specificity, accuracy and stability. The QuicKey ELISA^® Kit guarantees the experiment performance and greatly improve the detection efficiency!

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	Traditional ELISA	QuicKey ELISA^®	What it means for you
Assay Time	3.5h	2.5h	saving 1h
Assay Procedure	7 steps	6 steps	remove the calculation and dilution process of Biotinylated Detection Ab, add it directly
Storage	different components should be separately stored at -20℃ or 4℃	all components are stored at 4℃	same storage condition, avoid affecting the validity period due to improper storage
Difference Between Biotinylated Detection Ab and Concentrated HRP Conjugate	both are concentrated, distinguished by the color of the cap, the working solutions are colorless	Biotinylated Detection Ab is colorless, HRP Conjugate working solution is red	less risk of mistakes

How Does QuicKey ELISA^® Work?

QuicKey ELISA^®

	1. Add 100μL standard or sample to each well. Immediately add 100 μL Biotinylated Detection Ab to each well. Incubate for 90 min at 37℃.
	2. Aspirate and wash 3 times.
	3. Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.
	4. Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃.
	5. Add 50 μL Stop Solution.
	6. Read at 450nm immediately. Calculation of results.

Traditional Sandwich ELISA

	1. Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.
	2. Remove the liquid.
	3. Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.
	4. Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.
	5. Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃.
	6. Add 50 μL Stop Solution.
	7. Read at 450 nm immediately. Calculation of results.