QuicKey ELISA® Kits
QuicKey ELISA® Kit, is an improvement of the traditional sandwich ELISA Kit, it simplifies the operation process, and only 2 washing steps and 3 incubation steps are required. It shortens the experiment time from 3.5 hours to 2.5 hours without compromising high sensitivity, specificity, accuracy and stability. The QuicKey ELISA® Kit guarantees the experiment performance and greatly improve the detection efficiency!
|Traditional ELISA||QuicKey ELISA®||What it means for you|
|Assay Time||3.5h||2.5h||saving 1h|
|Assay Procedure||7 steps||6 steps||remove the calculation and dilution process of Biotinylated Detection Ab, add it directly|
|Storage||different components should be separately stored at -20℃ or 4℃||all components are stored at 4℃||same storage condition, avoid affecting the validity period due to improper storage|
|Difference Between Biotinylated Detection Ab and Concentrated HRP Conjugate||both are concentrated, distinguished by the color of the cap, the working solutions are colorless||Biotinylated Detection Ab is colorless, HRP Conjugate working solution is red||less risk of mistakes|
How Does QuicKey ELISA® Work?
- QuicKey ELISA®
1. Add 100μL standard or sample to each well. Immediately add 100 μL Biotinylated Detection Ab to each well. Incubate for 90 min at 37℃. 2. Aspirate and wash 3 times. 3. Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times. 4. Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃. 5. Add 50 μL Stop Solution. 6. Read at 450nm immediately. Calculation of results.
- Traditional Sandwich ELISA
1. Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃. 2. Remove the liquid. 3. Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times. 4. Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times. 5. Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃. 6. Add 50 μL Stop Solution. 7. Read at 450 nm immediately. Calculation of results.
Why Choose QuicKey ELISA® Kits？
Save 1h at least
More simplified assay procedures
Higher stability, easy storage
More cost-effective, lower price
Targets for research
- Inflammation Research
- IgG (Human)
- IgG (Mouse)
- TG(specific for thyroid)