QuicKey ELISA® Kits
QuicKey ELISA® Kit, is an improvement of the traditional sandwich ELISA Kit, it simplifies the operation process, and only 2 washing steps and 3 incubation steps are required. It shortens the experiment time from 3.5 hours to 2.5 hours without compromising high sensitivity, specificity, accuracy and stability. The QuicKey ELISA® Kit guarantees the experiment performance and greatly improve the detection efficiency!
Traditional ELISA | QuicKey ELISA® | What it means for you | |
---|---|---|---|
Assay Time | 3.5h | 2.5h | saving 1h |
Assay Procedure | 7 steps | 6 steps | remove the calculation and dilution process of Biotinylated Detection Ab, add it directly |
Storage | different components should be separately stored at -20℃ or 4℃ | all components are stored at 4℃ | same storage condition, avoid affecting the validity period due to improper storage |
Difference Between Biotinylated Detection Ab and Concentrated HRP Conjugate | both are concentrated, distinguished by the color of the cap, the working solutions are colorless | Biotinylated Detection Ab is colorless, HRP Conjugate working solution is red | less risk of mistakes |
How Does QuicKey ELISA® Work?
- QuicKey ELISA®
-
1. Add 100μL standard or sample to each well. Immediately add 100 μL Biotinylated Detection Ab to each well. Incubate for 90 min at 37℃. 2. Aspirate and wash 3 times. 3. Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times. 4. Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃. 5. Add 50 μL Stop Solution. 6. Read at 450nm immediately. Calculation of results.
- Traditional Sandwich ELISA
-
1. Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃. 2. Remove the liquid. 3. Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times. 4. Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times. 5. Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃. 6. Add 50 μL Stop Solution. 7. Read at 450 nm immediately. Calculation of results.
Why Choose QuicKey ELISA® Kits?
Save 1h at least
More simplified assay procedures
Higher stability, easy storage
More cost-effective, lower price
Targets for research
- Inflammation Research
- IgA
- IgG (Human)
- IgG (Mouse)
- IgM
- IgE
- CRP
- TGF-α
- ADP/Acrp30
- ALB
- PCT
- TG(specific for thyroid)
- Signaling Transduction
- VEGF-A
- TGF-α
- ADP/Acrp30