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DDX39B Polyclonal Antibody

Cat.No.:E-AB-62958

Host: Rabbit

Reactivity: H,M,R

Applications: IHC,IF

Summary of Verified Samples

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Immunohistochemistry of paraffin-embedded Rat liver using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat ovary using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat brain using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat spleen using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Human appendix using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Human breast cancer using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

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Test Application

Verified Samples

Reactivity Application
Human IHC
(appendix,breast cancer,)

Immunohistochemistry of paraffin-embedded Human appendix using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Human breast cancer using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

IF
(U-2OS,)

Immunofluorescence analysis of U-2OS cells using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.

Rat IHC
(liver,ovary,brain,spleen,)

Immunohistochemistry of paraffin-embedded Rat liver using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat ovary using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat brain using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat spleen using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).

IF
(C6,)

Immunofluorescence analysis of C6 cells using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.

Mouse IF
(NIH/3T3,)

Immunofluorescence analysis of NIH/3T3 cells using DDX39B Polyclonal Antibody at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.

Dilution

IHC 1:100-1:200 IF 1:50-1:200

Related Reagents

Application Products
WB Western Blot Detection Kit(E-IR-R304)
IHC 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)(E-IR-R217)

Product Details

Isotype IgG
Concentration 1mg/mL
Storage Store at -20°C. Avoid freeze / thaw cycles.
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Affinity purification
Research Areas Cancer, Epigenetics and Nuclear Signaling
Conjugation Unconjugated
Cellular cellular_localization Nucleus. Nucleus speckle.

Immunogen Details

Immunogen Recombinant fusion protein of human DDX39B (NP_004631.1).
Abbre DDX39B
Synonyms DDX39B,BAT1,D6S81E,UAP56
Swissprot Q13838
Gene ID 7919

Background

This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene.
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