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NRP1 Polyclonal Antibody

Cat.No.:E-AB-66905

Host: Rabbit

Reactivity: H,M,R

Applications: WB,IF

Summary of Verified Samples

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Western blot analysis of extracts of various cell lines using NRP1 Polyclonal Antibody at dilution of 1:3000.

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Price:   $129
Lead Time:   7~10 days
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Test Application

Verified Samples

Reactivity Application
Human WB
(SH-SY5Y,A549,U87-MG,293T,)

Western blot analysis of extracts of various cell lines using NRP1 Polyclonal Antibody at dilution of 1:3000.

Western blot analysis of extracts of various cell lines using NRP1 Polyclonal Antibody at dilution of 1:3000.

Western blot analysis of extracts of various cell lines using NRP1 Polyclonal Antibody at dilution of 1:3000.

Western blot analysis of extracts of various cell lines using NRP1 Polyclonal Antibody at dilution of 1:3000.

IF
(U-2OS,)

Immunofluorescence analysis of U-2OS cells using NRP1 Polyclonal Antibody at dilution of 1:100.Blue: DAPI for nuclear staining.

Rat IF
(C6,)

Immunofluorescence analysis of C6 cells using NRP1 Polyclonal Antibody at dilution of 1:100.Blue: DAPI for nuclear staining.

Mouse WB
(brain,)

Western blot analysis of extracts of various cell lines using NRP1 Polyclonal Antibody at dilution of 1:3000.

IF
(L929,)

Immunofluorescence analysis of L929 cells using NRP1 Polyclonal Antibody at dilution of 1:100.Blue: DAPI for nuclear staining.

Dilution

WB 1:500-1:2000 IF 1:50-1:200

Related Reagents

Application Products
WB Western Blot Detection Kit(E-IR-R304)
IHC 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)(E-IR-R217)

Product Details

Isotype IgG
Concentration 1mg/mL
Storage Store at -20°C. Avoid freeze / thaw cycles.
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Affinity purification
Research Areas Cancer, Cardiovascular, Immunology, Neuroscience
Conjugation Unconjugated
Cellular cellular_localization Secreted and Cell membrane.

Immunogen Details

Immunogen Recombinant fusion protein of human NRP1 (NP_001019800.1).
Abbre NRP1
Synonyms BDCA4,CD304,NP1,NRP,VEGF165R,Neuropilin-1,NRP1
Swissprot O14786
Gene ID 8829
Calculated MW 68kDa/71kDa/103kDa
Observed MW 103kDa

Background

This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
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