Added to cart successfully !

QuicKey Human BMG/β2-MG(Beta-2-Microglobulin) ELISA Kit

Size:  
Qty:
- +
Price:   $390
Cat Number:  E-TSEL-H0004
Lead Time:   7~10 days

Reactivity:  Human

Detection Range:  0.78~50 ng/mL

Sensitivity:  0.47 ng/mL

Add to cart

Product Details

QuicKey Series

Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 2.5 h
Reactivity Human
Detection method Colormetric
Colormetric 0.78-50 ng/mL
Sensitivity 0.47 ng/mL
Sample volume 50μL/well
Sample type serum, plasma, urine
Specificity This kit recognizes Human BMG/β2-MG in samples. No significant cross-reactivity or interference between Human BMG/β2-MG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human BMG/β2-MG concentrations in serum, plasma, urine. Please consult technical support for the applicability if other biological fluids need to be tested.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMG/β2-MG. Samples (or Standards) and biotinylated detection antibody specific for Human BMG/β2-MG are added to the micro ELISA plate wells. Human BMG/β2-MG would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMG/β2-MG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human BMG/β2-MG. You can calculate the concentration of Human BMG/β2-MG in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below. (The specific components might be slightly different in delivered kits, please refer to the manual for more information.)

Item Specifications Storage conditions after test
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
2-8℃, 1 month
Reference Standard 96T: 2 vials
48T: 1 vial
Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃
Biotinylated Detection Ab Working Solution 1 vial, 6 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
2-8℃ (Protect from light)
Substrate Reagent 1 vial, 10 mL
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

1. Microplate reader with 450nm wavelength filter

2. High-precision transfer pipette, EP tubes and disposable pipette tips

3. Incubator capable of maintaining 37℃

4. Deionized or distilled water

5. Absorbent paper

6. Loading slot for Wash Buffer

Technical Data

Sample values

sandwich-Ab-ELISA-Elabscience

Typical Standard Curve

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL) 50 25 12.5 6.25 3.13 1.56 0.78 0
OD 2.483 1.659 0.959 0.478 0.273 0.187 0.125 0.06
Corrected OD 2.423 1.599 0.899 0.418 0.213 0.127 0.065 -
sandwich-Ab-ELISA-Elabscience

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human BMG/β2-MG were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human BMG/β2-MG were tested on 3 different plates, 20 replicates in each plate.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(ng/mL) 2.35 5.89 24.08 2.16 5.00 22.71
Standard deviation 0.13 0.27 1.17 0.13 0.25 1.22
CV (%) 5.53 4.58 4.86 6.02 5.00 5.37

Recovery

The recovery of Human BMG/β2-MG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-105 99
EDTA plasma(n=8) 92-104 98
Urine(n=8) 97-103 100

Linearity

Samples were spiked with high concentrations of Human BMG/β2-MG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum(n=4) EDTA plasma (n=4) Urine(n=4)
1:2 Range (%) 97-105 96-110 95-105
Average (%) 100 102 101
1:4 Range (%) 86-96 84-96 97-113
Average (%) 93 91 105
1:8 Range (%) 94-101 98-109 102-112
Average (%) 97 102 105
1:16 Range (%) 93-104 89-101 97-107
Average (%) 98 94 101

Target information

Background

Beta 2 microglobulin (β2M) is a small protein (11,800 Dalton), normally found on the surface of many cells, including lymphocytes, and in small amounts in the blood and urine [1]. An increased amount in the blood or urine may be a sign of certain diseases, including some types of cancer, such as multiple myeloma or lymphoma.β2M is critical for the immune surveillance and modulation in vertebrate animals. The dysregulation of β2M is associated with multiple diseases, including endogenous and infectious diseases. β2M could directly participate in the development of cancer cells, and the level of β2M is deemed as a prognostic marker for several malignancies. It also involves in forming major histocompatibility complex (MHC class I or MHC I) or like heterodimers, covering from antigen presentation to immune homeostasis [2].
1. Güssow D, Rein R, Ginjaar I, et al. The human beta 2-microglobulin gene. Primary structure and definition of the transcriptional unit. [J]. Journal of Immunology, 1987, 139(9):3132-8.
2. Ohta Y, Shiina T, Lohr R L, et al. Primordial linkage of β2-microglobulin to the MHC [J]. Journal of Immunology, 2011, 186(6):3563-3571.

Research area

Database Links

SwissProt P61769 Human
Entrez Gene 567 Human

Synonyms

B2MG, B2M, β2M

Documents

Citations

Please kindly contact us if you have published a paper by citing any products from Elabscience, we will get back to you with the Elabscience Publication Reward ASAP.

Review more published papers in our Publication Library

Assay procedures

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results
If you have any question about the product, please feel free to contact us or leave a message