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QuicKey Human TG(Thyroglobulin) ELISA Kit

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Price:   $390
Cat Number:  E-TSEL-H0025
Lead Time:   7~10 days

Reactivity:  Human

Detection Range:  15.63~1000 pg/mL

Sensitivity:  3.38 pg/mL

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Product Details

QuicKey Series

Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 2.5 h
Reactivity Human
Detection method Colormetric
Colormetric 15.63-1000 pg/mL
Sensitivity 3.38 pg/mL
Sample volume 50μL/well
Sample type serum, plasma
Specificity This kit recognizes Human TG in samples. No significant cross-reactivity or interference between Human TG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human TG concentrations in serum, plasma. Please consult technical support for the applicability if other biological fluids need to be tested.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TG. Samples (or Standards) and biotinylated detection antibody specific for Human TG are added to the micro ELISA plate wells. Human TG would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human TG. You can calculate the concentration of Human TG in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below. (The specific components might be slightly different in delivered kits, please refer to the manual for more information.)

Item Specifications Storage conditions after test
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
2-8℃, 1 month
Reference Standard 96T: 2 vials
48T: 1 vial
Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃
Biotinylated Detection Ab Working Solution 1 vial, 6 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
2-8℃ (Protect from light)
Substrate Reagent 1 vial, 10 mL
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

1. Microplate reader with 450nm wavelength filter

2. High-precision transfer pipette, EP tubes and disposable pipette tips

3. Incubator capable of maintaining 37℃

4. Deionized or distilled water

5. Absorbent paper

6. Loading slot for Wash Buffer

Technical Data

Sample values


Typical Standard Curve

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL) 1000 500 250 125 62.5 31.25 15.63 0
OD 2.3355 1.68 1.3155 0.742 0.4305 0.2645 0.179 0.08
Corrected OD 2.2555 1.6 1.2355 0.6625 0.3505 0.1845 0.098 -


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TG were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TG were tested on 3 different plates, 20 replicates in each plate.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 47.23 118.63 485.67 42.51 124.90 446.45
Standard deviation 2.81 5.43 23.94 2.36 5.96 23.97
CV (%) 5.95 4.58 4.93 5.55 4.77 5.37


The recovery of Human TG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 98-110 103
EDTA plasma(n=8) 96-106 103


Samples were spiked with high concentrations of Human TG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum(n=4) EDTA plasma (n=4)
1:2 Range (%) 95-106 87-100
Average (%) 98 92
1:4 Range (%) 88-103 94-109
Average (%) 96 102
1:8 Range (%) 91-104 98-104
Average (%) 96 101
1:16 Range (%) 99-112 92-100
Average (%) 104 96

Target information


Thyroglobulin (Tg) is a large glycoprotein that is stored in follicles in healthy thyroid tissue as colloid where it acts as a substrate for thyroid hormones synthesis. Thyroglobulin is a homologous dimer with a molecular weight of about 660 kDa. Human protein consists of 2,767 amino acid residues and is the storage form of iodine in the thyroid gland, precursor of the iodinated thyroid hormones, thyroxine (T4) and triiodothyronine (T3). It is exclusively produced by normal or well-differentiated malignant thyrocytes. Then, its tissue-specific origin makes it eminently suitable as a serum tumor indicator in patients followed-up for differentiated thyroid carcinoma (DTC) after thyroid removal and remnants ablation[1].The most studied polymorphisms in the TG gene are rs2069550 (E10SNP158), rs853326 (E12SNP), and rs180223 (E10SNP24) in exons 10–12 and rs2076740 (E33SNP) in exon 33 SNP, which were reported to be associated with AITD[2].
1. Grebe S K G. Diagnosis and management of thyroid carcinoma: a focus on serum thyroglobulin[J]. Expert Review of Endocrinology & Metabolism, 2009, 4(1): 25-43.
2. Wang L Q, Wang T Y, Sun Q L, et al. Correlation between thyroglobulin gene polymorphisms and autoimmune thyroid disease[J]. Molecular medicine reports, 2015, 12(3): 4469-4475.

Research area

Database Links

SwissProt P01266 Human
Entrez Gene 7038 Human





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Assay procedures

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results
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