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QuicKey Pro Human E2(Estradiol) ELISA Kit

E-OSEL-H0005

Size:   Price:   $520
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QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 31.25-2000 pg/mL
Sensitivity 13.22 pg/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E2 are added to the micro ELISA plate wells. Human E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T
2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials
2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL
2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL
2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level E2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level E2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 9.73 25.19 80.21 10.31 25.61 86.43
Standard deviation 0.51 1.26 4.24 0.68 1.19 4.61
CV (%) 5.26 4.99 5.29 6.57 4.66 5.33

Recovery

The recovery of E2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-98 92
EDTA plasma(n=8) 86-101 93
Urine(n=8) 99

Linearity

Samples were spiked with high concentrations of Human E2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsOestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol
Research AreaCell biology, Metabolism

Assay Procedures

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