QuicKey Pro Human ICAM-1/CD54(intercellular adhesion molecule 1) ELISA Kit
E-OSEL-H0018
Size: | Price: | $520 | |
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QuicKey Pro Series
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Product Details
Properties
Assay type | Sandwich-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 0.25-16 ng/mL |
Sensitivity | 0.15 ng/mL |
Sample type &Sample volume | ; 50μL |
Specificity | This kit recognizes ICAM-1 in samples. No significant cross-reactivity or interference between ICAM-1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of ICAM-1 concentrations in . |
Test Principle
Kit components & Storage
Item | Specifications | Storage |
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Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
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Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
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Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
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Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Technical Data
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ICAM-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ICAM-1 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 0.86 | 2.24 | 5.52 | 0.87 | 2.28 | 5.82 |
Standard deviation | 0.05 | 0.09 | 0.25 | 0.06 | 0.10 | 0.19 |
CV (%) | 5.97 | 4.11 | 4.47 | 6.35 | 4.30 | 3.18 |
Recovery
The recovery of ICAM-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 88-104 | 96 |
EDTA plasma(n=8) | 87-99 | 94 |
Cell culture media(n=8) | 91-104 | 96 |
Linearity
Samples were spiked with high concentrations of Human ICAM-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Target Information
Database Links | SwissProt: P05362 |
Synonyms | CD54 |
Research Area | Cancer, Cardiovascular, Immunology, Stem cells |