QuicKey Pro Human IL-6(Interleukin 6) ELISA Kit
E-OSEL-H0001
| Size: | Price: | $520 | |
| Qty: |
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QuicKey Pro Series
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Product Details
Properties
| Assay type | Sandwich-ELISA |
| Format | 96T/48T/24 |
| Assay time | 1.5h |
| Detection range | 3.13-200 pg/mL |
| Sensitivity | 1.02 pg/mL |
| Sample type &Sample volume | serum and plasma; 50μL |
| Specificity | This kit recognizes IL-6 in samples. No significant cross-reactivity or interference between IL-6 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Application | This ELISA kit applies to the in vitro quantitative determination of IL-6 concentrations in serum and plasma. |
Test Principle
Kit components & Storage
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
| Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
| Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
| HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
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| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
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| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
| Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
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| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Technical Data
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IL-6 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IL-6 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 9.10 | 22.29 | 90.92 | 9.12 | 21.98 | 90.28 |
| Standard deviation | 0.57 | 1.13 | 3.32 | 0.53 | 1.31 | 3.25 |
| CV (%) | 6.24 | 5.06 | 3.65 | 5.84 | 5.98 | 3.60 |
Recovery
The recovery of IL-6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-102 | 96 |
| EDTA plasma(n=8) | 92-105 | 97 |
Linearity
Samples were spiked with high concentrations of Human IL-6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Target Information
| Database Links | SwissProt: P05231 |
| Synonyms | Interleukin 6,IL6, BSF2, HGF, HSF, IFNB2, CDF |
| Research Area | Cancer, Metabolism, Immunology, Neuroscience |
Assay Procedures
