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Recombinant Human TFPI Protein (His Tag)

PKSH031525

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Product Details

Catalog number PKSH031525
Expression_host HEK293 Cells
Sequence Met 1-Lys 282
Bio_Activity Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114). The IC50 value is < 0.4 nM, as measured in 100μL reaction mixture containing 1. 25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.
Accession NP_006278.1
Synonyms EPI;LACI;TFI;TFPI1
Tag C-His
Purity > 85 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Formulation Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
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